cDNA Clone IKK epsilon

• Plasmid: pCMV-FLAG-1 IKK epsilon K38A
• Parent Plasmid: pCMV5 HA1
• Expressed: FLAG-IKK epsilon K38A
• Genbank: NP_054721
• Tag: FLAG

Sequence of Insert

GGATCCATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCT
GGGGCAGGGGGCCACTGCCAGTGTGTACAAGGCCCGCAACAAGAAATCCG
GAGAGCTGGTTGCTGTGGCGGTCTTCAACACTACCAGCTACCTGCGGCCC
CGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCA
GAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGA
AGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTG
GAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGT
GCTGCGCTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTG
TGCATCGCGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAG
GGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGCTGGA
TGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATC
CCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTC
GGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGC
CACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGG
AGATCATGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGT
GCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCAT
CACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATCCTGG
CCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTC
TTTGCGGAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTC
CCTGTCCCAGGCAGTCCTGCACCACATCTATATCCATGCCCACAACACGA
TAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGA
CACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGT
CTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCT
TCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGAC
GTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGC
CAAGGGCGTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCC
TGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAG
GTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCT
CCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTG
GAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGG
CTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGA
GACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGG
ATCAGGTACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGAC
AAGATGAACTTCATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGG
GCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCA
GTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAG
AAGTATCAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCA
CGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTA
ACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAG
CTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCC
GCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCC
ACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTG
GACAACAACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGA
TGTCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVAV
FNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYC
SSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPG
NIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAV
LRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITT
EKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQ
AKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAV
HKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKG
LAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELM
FRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQEL
KAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRS
IQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLL
QVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQ
ESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEG
MKLLASDLLDNNRIIERLNRVPAPPDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12713
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression. Kinase Dead mutation.; ; Estimated MW of expressed protein : 81672 Daltons