cDNA Clone IRAK1

• Plasmid: pCMV-HA-2 IRAK1 K242R K253R K355R K397R
• Parent Plasmid: pCMV5 HA2
• Expressed: HA IRAK1 K242R K253R K355R K397R
• Genbank: AAC41949
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGCAGCCCCCGG
CGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCT
ACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCCGCC
CTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCA
GCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACGCCCGTGTGG
CCGACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGAC
ATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCCAGGCACCAC
TGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGA
GCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCCCCAGCTTTT
CCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCC
TGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTTCTACCAAGC
CAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTCTCCG
TTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGA
GGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGA
TGAGGAACACGGTGTATGCTGTGAAGAGGCTGAGGGAGAACGCTGACCTG
GAGTGGACTGCAGTGAGGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTC
CAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGA
ACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAG
GACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCA
GCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATC
AGGACAGCCCCAGCCTCATCCATGGAGACATCAGGAGTTCCAACGTCCTT
CTGGATGAGAGGCTGACACCCAGGCTGGGAGACTTTGGCCTGGCCCGGTT
CAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGA
CACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAGG
ACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGT
GCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGA
CCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTG
GCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGC
CTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGACCCCA
GGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCC
TGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGT
GTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGC
ATTTGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTAC
GTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCT
GGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAG
GCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCT
GCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACC
CCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCT
GGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTT
GGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCC
TGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCC
TGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTG
GAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGAGC
GGCCGC

Amino Acid Sequence

Protein sequence. 1M of IRAk1 is 24M of expressed protein. HA tag is underlined. MYPYDVPDYAGIPGIPGSTRAAAMAGGPGPGEPAAPGAQHFLYEVPPWVM
CRFYKVMDALEPADWCQFAALIVRDQTELRLCERSGQRTASVLWPWINRN
ARVADLVHILTHLQLLRARDIITAWHPPAPLPSPGTTAPRPSSIPAPAEA
EAWSPRKLPSSASTFLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPS
STKPGPESSVSLLQGARPSPFCWPLCEISRGTHNFSEELKIGEGGFGCVY
RAVMRNTVYAVKRLRENADLEWTAVRQSFLTEVEQLSRFRHPNIVDFAGY
CAQNGFYCLVYGFLPNGSLEDRLHCQTQACPPLSWPQRLDILLGTARAIQ
FLHQDSPSLIHGDIRSSNVLLDERLTPRLGDFGLARFSRFAGSSPSQSSM
VARTQTVRGTLAYLPEEYIRTGRLAVDTDTFSFGVVVLETLAGQRAVKTH
GARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKH
LDPRPGPCPPELGLGLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAG
VPGHLEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQ
LQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAG
ESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYE
DGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12629
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA tag for mammalian expression.; ; Estimated MW of expressed protein : 79144 Daltons