cDNA Clone Pellino3

• Plasmid: pGEX6P-1-Pellino3
• Parent Plasmid: pGEX6P1
• Expressed: GST-Pellino3
• Genbank: AAH21256.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGATGCTGTGAGGAAGGAGGTGAGGAAACCGAGGCTCAGAGAGG
GGAAGTGACTGGCCCAAGGGCACACAGCTGCTACAATGGTTGTCTGGCAA
GTGGGGACAAGGGCCGCCGGCGAAGCCGCCTGGCACTGAGCCGCCGGTCG
CACGCCAACGGGGTGAAGCCAGACGTCATGCACCACATCTCCACGCCGCT
CGTCTCCAAGGCACTGAGTAACCGTGGTCAGCACAGCATCTCGTATACAC
TGTCCCGGAGCCACTCGGTCATAGTGGAGTATACACATGATAGCGACACA
GACATGTTCCAGATTGGCCGCTCCACAGAGAACATGATTGACTTCGTGGT
AACAGACACGTCCCCTGGAGGAGGGGCTGCCGAGGGCCCTTCTGCCCAGA
GCACCATCTCCCGCTATGCCTGCCGCATCCTCTGTGACCGCCGGCCACCC
TATACTGCCCGCATCTATGCCGCTGGCTTCGACGCCTCTAGCAACATCTT
CCTTGGAGAGCGAGCGGCCAAATGGCGGACCCCAGATGGCCTGATGGATG
GACTGACCACCAATGGAGTCCTGGTGATGCACCCGGCAGGCGGCTTCTCC
GAGGACTCAGCCCCGGGTGTCTGGCGGGAGATCTCGGTCTGTGGGAATGT
GTACACATTGCGGGACAGCCGCTCAGCCCAGCAGCGGGGCAAGCTGGTAG
GTGGCCCGCTCCATTCCCCACCCCTATCCTTGCCAGGCCCTCACAAGCTG
CCCATCCCCCTGCCCAAGCCAGGAGATGATCTCCAGTCCTTCTGCCCCAG
GGAGCCCCAACAGGCTCCATATCAGGGAATCCCAGGCCCAGGGGGTTCTT
AGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVLEGNPEVGSPRTSDLQH
RGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQRGEVTG
PRAHSCYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHISTPLVSKA
LSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDFVVTDTS
PGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGER
AAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLR
DSRSAQQRGKLVGGPLHSPPLSLPGPHKLPIPLPKPGDDLQSFCPREPQQ
APYQGIPGPGGS

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12541
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed proteins : 62161 Daltons