cDNA Clone Pellino1

• Plasmid: pGEX6P-1-Pellino1 138-418
• Parent Plasmid: pGEX6P1
• Expressed: GST-Pellino1 138-418
• Genbank: Q96FA3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCTGCAGAATCATATGTGAACGGAATCCTCCCTTTACAGCACG
GATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTTTCTTGGGGAGA
AGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATGGCTTGACCACT
AATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACAGAAGACTCCAA
GCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGTATTTAGCCTAC
GTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGGAAATTGAAACC
AATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGTGCAACATTGTT
ATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGTGAAGCATTTAG
AAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGTGCCCTGTAGGG
TTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGACGTTGTAGATGA
AAAACAACCATGGGTATATCTAAACTGCGGCCATGTACATGGCTATCATA
ACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTGAATGTCCTATG
TGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGATGTGAAGCTGG
ATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAGCCCGTGTGGGC
ATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGATCCCACTTCCT
CATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGTGCACATCAGTT
GGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGGACCTCTAGACT
AAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSACRIICERNPPFTARIYAA
GFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIW
REISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTA
EGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPW
VYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVD
AGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQ
GYIRLIFQGPLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 58095 Daltons