cDNA Clone Pellino1

• Plasmid: pEBG6P-Pellino1 F11S
• Parent Plasmid: pEBG6P
• Expressed: GST Pellino1 F11S
• Genbank: Q96FA3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTTTCTCCTGATCAAGAAAATCATCCATCTAAAGCACCAGT
AAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATG
GCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAG
GCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGC
TGCAAAGGCAATAAGCAACAAAGACCAGCATAGCATATCATATACTTTGT
CTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACAGAT
ATGTTTCAAATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAAC
TGACACGGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAA
GCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCC
TTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTT
TCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATG
GCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACA
GAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGT
ATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGG
AAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGT
GCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGT
GAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGT
GCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGAC
GTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACA
TGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTG
AATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGA
TGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAG
CCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGA
TCCCACTTCCCCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGT
GCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGG
ACCTCTAGACTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFSPDQENHPSKA
PVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTP
QAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFV
VTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKN
IFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCG
NVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTP
TVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGH
VHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHA
FSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIF
QGPLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12485
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 73701 Daltons