cDNA Clone AMPKG3

• Synonyms: PRKAG3
• Plasmid: pGEX-4T-AMPKG3 197-478
• Parent Plasmid: pGEX4T1
• Expressed: GST AMPKg3 197-478
• Genbank: NM_017431
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCCGGATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCT
GGAGATCAAGAAGGCCTTCTTTGCTCTGGTGGCCAACGGTGTGCGGGCAG
CCCCTCTATGGGACAGCAAGAAGCAGAGCTTTGTGGGGATGCTGACCATC
ACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCCCCTGGTCCA
GATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACC
TGCAAGGCTGCTTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTG
TTTGAAGCTGTCTACACCCTCATCAAGAACCGGATCCATCGCCTGCCTGT
TCTTGACCCGGTGTCAGGCAACGTACTCCACATCCTCACACACAAACGCC
TGCTCAAGTTCCTGCACATCTTTGGTTCCCTGCTGCCCCGGCCCTCCTTC
CTCTACCGCACTATCCAAGATTTGGGCATCGGCACATTCCGAGACTTGGC
TGTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACATCTTTGTGG
ACCGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTG
GGCCTCTATTCCCGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTA
CAACCACCTGGACATGAGTGTGGGAGAAGCCCTGAGGCAGAGGACACTAT
GTCTGGAGGGAGTCCTTTCCTGCCAGCCCCACGAGAGCTTGGGGGAAGTG
ATCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTAGTGGACGA
GACCCAGCATCTCTTGGGCGTGGTCTCCCTCTCCGACATCCTTCAGGCAC
TGGTGTGACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFRMATSSKLVIFDTMLEIKKAF
FALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQ
HKIETWREIYLQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSG
NVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQDLGIGTFRDLAVVLETA
PILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMS
VGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLG
VVSLSDILQALV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12483
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Corrected missing stop in Hardy clone DU12224.; ; Estimated MW of expressed protein : 58785 Daltons