cDNA Clone AMPKG2

• Plasmid: pGEX6P-AMPKG2 198-525 new
• Parent Plasmid: pGEX6P1
• Expressed: GST-AMPKg2 198-525
• Genbank: NP_001035723
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGATGCTGGAGAAGCTGGAGTTCGAGGACGAAGCAGTAGAAGA
CTCAGAAAGTGGTGTTTACATGCGATTCATGAGGTCACACAAGTGTTATG
ACATCGTTCCAACCAGTTCAAAGCTTGTTGTCTTTGATACTACATTACAA
GTTAAAAAGGCCTTCTTTGCTTTGGTAGCCAACGGTGTCCGAGCAGCGCC
ACTGTGGGAGAGTAAAAAACAAAGTTTTGTAGGAATGCTAACAATTACAG
ATTTCATAAATATACTACATAGATACTATAAATCACCTATGGTACAGATT
TATGAATTAGAGGAACATAAAATTGAAACATGGAGGGAGCTTTATTTACA
AGAAACATTTAAGCCTTTAGTGAATATATCTCCAGATGCAAGCCTCTTCG
ATGCTGTATACTCCTTGATCAAAAATAAAATCCACAGATTGCCCGTTATT
GACCCTATCAGTGGGAATGCACTTTATATACTTACCCACAAAAGAATCCT
CAAGTTCCTCCAGCTTTTTATGTCTGATATGCCAAAGCCTGCCTTCATGA
AGCAGAACCTGGATGAGCTTGGAATAGGAACGTACCACAACATTGCCTTC
ATACATCCAGACACTCCCATCATCAAAGCCTTGAACATATTTGTGGAAAG
ACGAATATCAGCTCTGCCTGTTGTGGATGAGTCAGGAAAAGTTGTAGATA
TTTATTCCAAATTTGATGTAATTAATCTTGCTGCTGAGAAAACATACAAT
AACCTAGATATCACGGTGACCCAGGCCCTTCAGCACCGTTCACAGTATTT
TGAAGGTGTTGTGAAGTGCAATAAGCTGGAAATACTGGAGACCATCGTGG
ACAGAATAGTAAGAGCTGAGGTCCATCGGCTGGTGGTGGTAAATGAAGCA
GATAGTATTGTGGGTATTATTTCCCTGTCGGACATTCTGCAAGCCCTGAT
CCTCACACCAGCAGGTGCCAAACAAAAGGAGACAGAAACGGAGTGACTCG
AGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMLEKLEFEDEAVEDSESG
VYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWES
KKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFK
PLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQ
LFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISA
LPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVV
KCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPA
GAKQKETETE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12482
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Corrected missing stop from original Hardy Clone DU12223.; ; Estimated MW of expressed protein : 64402 Daltons