cDNA Clone AMPKG2

• Plasmid: pGEX4T-1-AMPKG2 198-525
• Parent Plasmid: pGEX4T1
• Genbank: NP_001035723.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGATGCTGGAGAAGCTGGAGTTCGAGGACGAAGCAGTAGAAGA
CTCAGAAAGTGGTGTTTACATGCGATTCATGAGGTCACACAAGTGTTATG
ACATCGTTCCAACCAGTTCAAAGCTTGTTGTCTTTGATACTACATTACAA
GTTAAAAAGGCCTTCTTTGCTTTGGTAGCCAACGGTGTCCGAGCAGCGCC
ACTGTGGGAGAGTAAAAAACAAAGTTTTGTAGGAATGCTAACAATTACAG
ATTTCATAAATATACTACATAGATACTATAAATCACCTATGGTACAGATT
TATGAATTAGAGGAACATAAAATTGAAACATGGAGGGAGCTTTATTTACA
AGAAACATTTAAGCCTTTAGTGAATATATCTCCAGATGCAAGCCTCTTCG
ATGCTGTATACTCCTTGATCAAAAATAAAATCCACAGATTGCCCGTTATT
GACCCTATCAGTGGGAATGCACTTTATATACTTACCCACAAAAGAATCCT
CAAGTTCCTCCAGCTTTTTATGTCTGATATGCCAAAGCCTGCCTTCATGA
AGCAGAACCTGGATGAGCTTGGAATAGGAACGTACCACAACATTGCCTTC
ATACATCCAGACACTCCCATCATCAAAGCCTTGAACATATTTGTGGAAAG
ACGAATATCAGCTCTGCCTGTTGTGGATGAGTCAGGAAAAGTTGTAGATA
TTTATTCCAAATTTGATGTAATTAATCTTGCTGCTGAGAAAACATACAAT
AACCTAGATATCACGGTGACCCAGGCCCTTCAGCACCGTTCACAGTATTT
TGAAGGTGTTGTGAAGTGCAATAAGCTGGAAATACTGGAGACCATCGTGG
ACAGAATAGTAAGAGCTGAGGTCCATCGGCTGGTGGTGGTAAATGAAGCA
GATAGTATTGTGGGTATTATTTCCCTGTCGGACATTCTGCAAGCCCTGAT
CCTCACACCAGCAGGTGCCAAACAAAAGGAGACAGAAACGGAGCCGCTCG
AG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSAMLEKLEFEDEAVEDSESGVYMRF
MRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSF
VGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNI
SPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSD
MPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVD
ESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKL
EILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQK
ETETEPLERPHRD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12223
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Contains an extra PLERPHRD due to no stop in insert.; ; Estimated MW of expressed protein : 64871 Daltons