cDNA Clone AMPKG3

• Synonyms: PRKAG3
• Plasmid: pGEX4T-1-AMPKG3 1-202
• Parent Plasmid: pGEX4T1
• Genbank: NP_059127.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTC
CTGGAGCAGCCTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAGAGC
AAGAAAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGAA
AGAATCCGTGGGAAACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAA
GTCGGTGGAGGAAGGGGAGCCACCAGGTCAGGGGGAAGGTCCCCGGTCCA
GGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCCCCAAGACCACA
CCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTG
GGACTGCCTCCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAG
ATGATGTGGAGCTGGCCACGGAGTTCCCAGCCACAGAGGCCTGGGAGTGT
GAGCTAGAAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGCCTGTCCCCGCA
GGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGGAAACCCGGCG
CCCAGATCTACATGCGCTTCATGCAGGAGCACACCTGCTACGATGCCATG
GCAACTAGCTCCAAGCCCGCTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSAMEPGLEHALRRTPSWSSLGGSEH
QEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGEPPG
QGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTA
SAAGSSTDDVELATEFPATEAWECELEGLLEERPALCLSPQAPFPKLGWD
DELRKPGAQIYMRFMQEHTCYDAMATSSKPARAQA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12222
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Contains PARAQA after last AMPKG3 sequence due to no stop in insert.; ; Estimated MW of expressed protein : 54255 Daltons