cDNA Clone PIP5K2B

• Plasmid: pFBHTb PI5P4 Kinase II beta
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PI5P4 Kinase II beta
• Genbank: 1BO1_A
• Tag: 6His

Sequence of Insert

GGATCCATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGGCGCC
GCTCAGCGCCAGCAAGACCAAGACCAAGAAGAAGCATTTCGTGTGCCAGA
AAGTGAAGCTATTCCGGGCCAGCGAGCCGATCCTCAGCGTCCTGATGTGG
GGGGTGAACCACACGATCAATGAGCTGAGCAATGTTCCTGTTCCTGTCAT
GCTAATGCCAGATGACTTCAAAGCCTACAGCAAGATCAAGGTGGACAATC
ATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTAAGTTTAAGGAGTAT
TGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGGAATTGATGATCA
GGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGACAGCC
AGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGACCGGCGCTTTGTC
ATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATCTTAAA
GAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTTGCCAC
AGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTGGAAACCTACATG
GTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGTGCATCGCAAGTA
TGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCGACAAGGAGAAGG
CCAAGGACTTGCCAACATTCAAAGACAATGACTTCCTCAATGAAGGGCAG
AAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTCCTGGAGAAACTGAA
GCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAGCCTGC
TGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGGAGGTG
GAGGAGCGGGCAGAGGACGAGGAGTGTGAGAATGATGGGGTGGGTGGCAA
CCTACTCTGCTCCTATGGCACACCTCCGGACAGCCCTGGCAACCTCCTCA
GCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCCTCTGTTGACGTC
TATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAGGAGGTGTATTTTAT
GGCCATCATTGATATCCTCACGCCATACGATACCAAGAAGAAAGCTGCAC
ATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGACTGTG
AACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAACATCCT
GACGTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSSNCTSTTAVAVAPLSASKTK
TKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFK
AYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVT
RSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIV
ECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTV
AREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLA
QLKIMDYSLLVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGT
PPDSPGNLLSFPRFFGPGEFDPSVDVYAMKSHESSPKKEVYFMAIIDILT
PYDTKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12477
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 50748 Daltons