cDNA Clone OBP

• Plasmid: pEBG6P-OBP human
• Parent Plasmid: pEBG6P
• Expressed: GST-OBP
• Genbank: NP_002547.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGACGGAGCTGAGAGGAGTGGTGGGGCCAGGCCCGGC
AGCCATTGCAGCACTTGGCGGCGGCGGCGCCGGTCCCCCAGTGGTGGGAG
GAGGCGGCGGCCGCGGAGATGCGGGGCCAGGCTCCGGGGCCGCGTCAGGG
ACGGTGGTCGCGGCGGCGGCGGGAGGCCCGGGCCCGGGGGCCGGGGGAGT
GGCGGCGGCTGGCCCGGCCCCTGCGCCGCCGACTGGGGGCTCGGGCGGCT
CGGGCGCTGGGGGTTCGGGCTCGGCTCGAGAGGGCTGGCTCTTCAAATGG
ACCAATTATATCAAAGGCTACCAGCGGCGATGGTTCGTGCTGAGCAACGG
GCTCCTGAGCTACTACAGATCAAAGGCAGAAATGAGACATACCTGCCGTG
GTACCATCAACCTCGCCACAGCCAACATCACCGTGGAGGACTCCTGCAAC
TTCATCATTTCCAATGGGGGTGCTCAGACCTACCATCTGAAAGCTAGTTC
AGAAGTTGAGCGGCAGCGCTGGGTGACGGCCCTGGAACTGGCCAAGGCCA
AAGCTGTGAAGATGCTGGCAGAGTCAGATGAATCAGGAGATGAAGAGTCT
GTCTCACAAACTGACAAGACTGAGCTGCAGAATACCCTTCGGACCCTCTC
TAGCAAAGTAGAGGACTTGAGCACGTGCAATGACTTGATAGCTAAGCATG
GCACAGCTCTGCAGCGTTCTCTCAGTGAGCTGGAGTCCCTGAAGTTGCCT
GCTGAGAGCAATGAAAAGATCAAACAGGTCAACGAACGAGCCACACTCTT
TAGGATAACATCCAATGCCATGATCAACGCCTGCAGAGATTTCCTCATGT
TAGCCCAGACCCATAGTAAAAAATGGCAAAAGTCACTACAGTATGAAAGA
GACCAGCGTATCCGACTGGAAGAAACCCTCGAGCAGCTGGCGAAGCAGCA
TAATCACCTGGAGAGGGCCTTCCGAGGAGCCACGGTGCTGCCGGCAAACA
CTCCTGGCAATGTGGGTTCTGGTAAAGATCAGTGCTGCTCTGGCAAAGGG
GACATGAGCGATGAAGATGATGAGAATGAATTTTTTGATGCACCTGAGAT
CATCACCATGCCTGAAAATTTGGGCCACAAACGTACTGGCAGCAATATCA
GTGGAGCCAGCAGTGACATCAGCCTTGATGAACAGTACAAGCATCAGCTG
GAGGAGACCAAAAAGGAAAAGAGAACCAGAATACCATACAAGCCAAACTA
TAGCCTCAATTTATGGAGCATCATGAAGAACTGCATTGGAAAAGAACTCT
CTAAGATCCCCATGCCGGTAAACTTTAATGAGCCCTTGTCCATGCTTCAG
CGCCTTACTGAAGATCTGGAATACCATGAGCTGTTAGACCGAGCTGCAAA
ATGTGAGAATTCTCTAGAACAGCTCTGTTATGTTGCAGCTTTCACCGTGT
CCTCCTACTCCACTACTGTCTTCCGCACCAGTAAGCCATTCAACCCACTG
CTTGGGGAGACCTTTGAGCTGGACCGATTAGAGGAGAATGGGTACCGATC
CCTCTGTGAACAGGTGAGTCATCATCCCCCTGCTGCTGCGCACCATGCTG
AGTCCAAAAATGGCTGGACATTGCGTCAGGAAATCAAAATCACCAGCAAG
TTTCGAGGCAAATACCTCTCCATTATGCCCCTCGGTACCATTCATTGTAT
TTTCCATGCAACTGGGCACCACTACACTTGGAAGAAAGTTACCACAACTG
TACACAACATTATTGTGGGCAAGTTGTGGATAGATCAGTCTGGCGAAATT
GATATTGTGAATCACAAGACAGGAGACAAGTGTAATCTTAAATTTGTTCC
TTATAGCTACTTCTCTCGGGATGTAGCAAGAAAGGTGACGGGGGAAGTGA
CAGATCCATCAGGAAAAGTCCACTTTGCTCTTCTGGGGACGTGGGATGAG
AAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGGGAAAATGGGGGTGA
TGCTCGACAGAGAGGCCATGAAGCAGAGGAAAGCAGGGTCATGCTGTGGA
AAAGGAATCCTTTACCGAAGAATGCAGAAAACATGTACTACTTCTCAGAG
CTTGCTCTGACTCTCAATGCTTGGGAAAGTGGCACTGCCCCCACAGACAG
CCGGTTACGACCTGACCAGAGACTGATGGAAAATGGACGCTGGGATGAAG
CAAATGCGGAGAAGCAGCGCCTGGAGGAAAAACAAAGACTTTCCAGAAAG
AAGAGAGAAGCGGAAGCTATGAAAGCCACAGAGGATGGCACACCATATGA
TCCCTATAAGGCACTGTGGTTTGAGCGGAAGAAGGACCCTGTTACCAAGG
AGTTAACCCATATTTATAGGGGAGAATACTGGGAGTGTAAAGAAAAACAG
GACTGGAGCTCATGCCCGGACATTTTCTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAATELRGVVGPG
PAAIAALGGGGAGPPVVGGGGGRGDAGPGSGAASGTVVAAAAGGPGPGAG
GVAAAGPAPAPPTGGSGGSGAGGSGSAREGWLFKWTNYIKGYQRRWFVLS
NGLLSYYRSKAEMRHTCRGTINLATANITVEDSCNFIISNGGAQTYHLKA
SSEVERQRWVTALELAKAKAVKMLAESDESGDEESVSQTDKTELQNTLRT
LSSKVEDLSTCNDLIAKHGTALQRSLSELESLKLPAESNEKIKQVNERAT
LFRITSNAMINACRDFLMLAQTHSKKWQKSLQYERDQRIRLEETLEQLAK
QHNHLERAFRGATVLPANTPGNVGSGKDQCCSGKGDMSDEDDENEFFDAP
EIITMPENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRTRIPYKP
NYSLNLWSIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYHELLDRA
AKCENSLEQLCYVAAFTVSSYSTTVFRTSKPFNPLLGETFELDRLEENGY
RSLCEQVSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLGTIH
CIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKF
VPYSYFSRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIGENG
GDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPT
DSRLRPDQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTP
YDPYKALWFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12459
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 116833 Daltons