cDNA Clone TRIM28

• Plasmid: pGEX6P-2-TRIM 28
• Parent Plasmid: pGEX6P2
• Expressed: GST-TRIM 28
• Genbank: NP_005753.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTC
GGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAA
AGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCG
GCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGC
TGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCC
GCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGT
GGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGG
AGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAG
GATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGC
ACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCA
GCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCA
TGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAG
ACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGAT
GCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAA
TCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATG
GCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGT
CAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGC
AGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGC
TTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGG
ATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCC
TGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGG
CACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGC
CCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAA
GGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCC
TTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTC
ACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCAC
TGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTA
CCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTG
GCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTC
TTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGC
AGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCC
AGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCT
GCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTC
CACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCA
GCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCA
CCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCA
CGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCC
TGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTC
CAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAG
ACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAAC
GAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCC
GATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCC
CTGGTGATGGCCCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAASAAAA
SAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEA
LELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGA
AGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCED
NAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVY
CNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLAS
LVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNK
RGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNN
TALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKI
VAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPY
SSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKV
FPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEET
EAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAP
EGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQD
VPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVL
LALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSP
QEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAV
LVEPPPMSLPGAGLSSQELSGGPGDGP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 116346 Daltons