cDNA Clone TRIM25

• Plasmid: pGEX6P-2-TRIM 25
• Parent Plasmid: pGEX6P2
• Expressed: GST-TRIM 25
• Genbank: NP_005073.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC
CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA
ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA
TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA
CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC
TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT
GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC
CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC
TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG
CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG
GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG
TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC
CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA
CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC
GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA
ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA
GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC
TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG
AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT
GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC
ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG
CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA
CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA
AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT
GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA
TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC
CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG
TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA
CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG
TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC
TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG
GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG
GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC
TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA
CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC
TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC
CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC
TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT
AGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAELCPLA
EELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQ
ARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACD
HCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCS
QHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTV
MYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETT
STRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFL
EKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEL
TPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGA
PEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIK
VILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYK
KGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKI
SAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFT
EALYPAFWVFSAGATLSICSPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12449
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 98789 Daltons