cDNA Clone AMPD2

• Plasmid: pEBG6P-AMPD2
• Parent Plasmid: pEBG6P
• Expressed: GST-AMPD2
• Genbank: Q9DBT5
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCTCAGAAGCTCGAAGCGGTCTGGGGGCCTCTCCGCTACA
GTCTGCCCGATCCCTGCCAGGCAACGCCCCTTGCCTCAAGCACTTCCCGC
TTGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAGATCGCTGAGGAG
TTGTTCAGCCGCTCACTGGCTGAGAGTGAGCTTCGTAGCGCCCCTTATGA
GTTCCCAGAGGAAAGCCCCATCGAGCAGCTAGAAGAACGGAGGCAGCGGC
TGGAGCGCCAGATCAGCCAGGATGTCAAGCTGGAGCCAGATATTCTTCTT
CGAGCCAAGCAAGATTTCCTGAAGACAGACAGCGACTCAGACTTACAGCT
GTACAAGGAGCAAGGAGAGGGACAGGGTGACAGGGGTCTTTGGGAACGTG
ATGTGGTATTGGAACGGGAATTTCAGCGGGTCATCATCTCTGGGGAGGAG
AAGTGTGGGGTGCCATTCACAGACCTCTTAGACGCAGCCAAAAGTGTGGT
TCGGGCACTTTTCATCCGGGAGAAGTACATGGCCCTATCACTGCAGAGCT
TCTGTCCCACCACCCGCCGTTACCTGCAGCAGCTGGCTGAGAAGCCCCTG
GAGACTCGAACTTATGAGCAGAGTCCTGATACCCCTGTATCTGCTGATGC
CCCAGTGCATCCCCCTGCACTGGAGCAGCACCCGTATGAGCACTGTGAGC
CAAGCGCCATGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGTGGT
GTGGTGCACGTCTACACCCGCAGGGACCCTGATGAGCACTGTCCGGAGGT
GGAGCTTCCATACCCTGACCTACAGGAATTTGTAGCTGACGTCAATGTGC
TGATGGCCCTGATCATCAATGGTCCCATAAAGTCATTCTGCTACCGCCGG
CTGCAGTACCTGAGCTCCAAATTCCAGATGCACGTTTTGCTCAATGAGAT
GAAGGAGCTCGCTGCTCAGAAGAAAGTGCCACACCGGGACTTCTACAATA
TCCGTAAGGTGGACACACACATCCACGCCTCGTCCTGCATGAACCAGAAA
CATCTACTGCGCTTCATCAAGCGGGCCATGAAGCGGCACCTGGAGGAGAT
TGTGCATGTGGAACAGGGCCGCGAGCAGACGCTGAGAGAAGTCTTCGAGA
GCATGAACCTCACTGCCTACGACTTAAGTGTGGACACGCTGGATGTGCAT
GCGGACAGGAATACCTTTCATCGATTTGACAAATTCAATGCCAAATACAA
CCCTATTGGGGAGTCTGTTCTCCGAGAGATCTTCATTAAAACCGACAACA
AGATTTCTGGGAAGTACTTTGCTCACATCATCAAGGAGGTGATGGCAGAC
TTGGAGGAGAGCAAATACCAGAATGCAGAGCTCCGGCTGTCCATCTACGG
GCGTTCGAGGGATGAGTGGGACAAGCTGGCACGCTGGGCAGTGAACCACA
AAGTGCACTCTCCCAATGTCCGCTGGCTGGTGCAGGTGCCCCGCCTCTTC
GATGTGTACCGCACCAAGGGCCAGCTGGCCAACTTCCAAGAGATGCTGGA
GAACATCTTTCTGCCCCTGTTTGAGGCTACTGTGCACCCTGCCAGCCACC
CGGAGCTGCACCTCTTTCTGGAGCACGTGGATGGTTTTGATAGCGTGGAT
GATGAGTCCAAGCCAGAGAACCACGTCTTCAACCTGGAGAGTCCCCTCCC
AGAAGCTTGGGTGGAGGAGGACAACCCTCCCTATGCCTACTACCTGTACT
ACACCTTCGCTAACATGGCTATGTTGAACCATCTGCGCAGGCAGAGAGGT
TTCCACACGTTCGTGCTGAGGCCGCACTGTGGGGAGGCCGGGCCCATCCA
CCACCTGGTATCAGCCTTCATGCTGGCCGAGAACATCTCCCACGGGCTGC
TCCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTATTACCTGGCTCAGATC
GGCATCGCCATGTCCCCGCTCAGCAACAACAGCCTGTTCCTCAGCTACCA
CCGGAACCCTCTCCCTGAGTACTTGTCCCGTGGCCTCATGGTCTCGCTGT
CCACAGATGATCCCTTGCAGTTCCACTTCACCAAGGAGCCCCTGATGGAG
GAGTACAGCATCGCCACCCAGGTGTGGAAGCTCAGCTCCTGCGATATGTG
CGAGCTGGCCCGTAACAGCGTGCTCATGAGTGGCTTCTCTCACAAGGTGA
AAAGCCACTGGCTGGGACCCAACTATACCAAGGAGGGCCCTGAGGGCAAT
GATATCCGCCGTACCAACGTGCCAGACATCCGAGTGGGCTACCGCTATGA
GACGCTATGCCAGGAGCTGGCACTTATCACACAGGCCGTCCAAAGTGAGA
TGCTGGAGACCATCCCAGAGGAAGTGGGCATTGTCATGAGCCCAGGGCCT
TAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMASEARSGLGASP
LQSARSLPGNAPCLKHFPLDLRTSMDGKCKEIAEELFSRSLAESELRSAP
YEFPEESPIEQLEERRQRLERQISQDVKLEPDILLRAKQDFLKTDSDSDL
QLYKEQGEGQGDRGLWERDVVLEREFQRVIISGEEKCGVPFTDLLDAAKS
VVRALFIREKYMALSLQSFCPTTRRYLQQLAEKPLETRTYEQSPDTPVSA
DAPVHPPALEQHPYEHCEPSAMPGDLGLGLRMVRGVVHVYTRRDPDEHCP
EVELPYPDLQEFVADVNVLMALIINGPIKSFCYRRLQYLSSKFQMHVLLN
EMKELAAQKKVPHRDFYNIRKVDTHIHASSCMNQKHLLRFIKRAMKRHLE
EIVHVEQGREQTLREVFESMNLTAYDLSVDTLDVHADRNTFHRFDKFNAK
YNPIGESVLREIFIKTDNKISGKYFAHIIKEVMADLEESKYQNAELRLSI
YGRSRDEWDKLARWAVNHKVHSPNVRWLVQVPRLFDVYRTKGQLANFQEM
LENIFLPLFEATVHPASHPELHLFLEHVDGFDSVDDESKPENHVFNLESP
LPEAWVEEDNPPYAYYLYYTFANMAMLNHLRRQRGFHTFVLRPHCGEAGP
IHHLVSAFMLAENISHGLLLRKAPVLQYLYYLAQIGIAMSPLSNNSLFLS
YHRNPLPEYLSRGLMVSLSTDDPLQFHFTKEPLMEEYSIATQVWKLSSCD
MCELARNSVLMSGFSHKVKSHWLGPNYTKEGPEGNDIRRTNVPDIRVGYR
YETLCQELALITQAVQSEMLETIPEEVGIVMSPGP

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12447
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 119438 Daltons