cDNA Clone AMPD2

• Plasmid: pGEX6P-1-AMPD2
• Parent Plasmid: pGEX6P1
• Expressed: GST-AMPD2
• Genbank: Q9DBT5
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCTCAGAAGCTCGAAGCGGTCTGGGGGCCTCTCCGCTACA
GTCTGCCCGATCCCTGCCAGGCAACGCCCCTTGCCTCAAGCACTTCCCGC
TTGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAGATCGCTGAGGAG
TTGTTCAGCCGCTCACTGGCTGAGAGTGAGCTTCGTAGCGCCCCTTATGA
GTTCCCAGAGGAAAGCCCCATCGAGCAGCTAGAAGAACGGAGGCAGCGGC
TGGAGCGCCAGATCAGCCAGGATGTCAAGCTGGAGCCAGATATTCTTCTT
CGAGCCAAGCAAGATTTCCTGAAGACAGACAGCGACTCAGACTTACAGCT
GTACAAGGAGCAAGGAGAGGGACAGGGTGACAGGGGTCTTTGGGAACGTG
ATGTGGTATTGGAACGGGAATTTCAGCGGGTCATCATCTCTGGGGAGGAG
AAGTGTGGGGTGCCATTCACAGACCTCTTAGACGCAGCCAAAAGTGTGGT
TCGGGCACTTTTCATCCGGGAGAAGTACATGGCCCTATCACTGCAGAGCT
TCTGTCCCACCACCCGCCGTTACCTGCAGCAGCTGGCTGAGAAGCCCCTG
GAGACTCGAACTTATGAGCAGAGTCCTGATACCCCTGTATCTGCTGATGC
CCCAGTGCATCCCCCTGCACTGGAGCAGCACCCGTATGAGCACTGTGAGC
CAAGCGCCATGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGTGGT
GTGGTGCACGTCTACACCCGCAGGGACCCTGATGAGCACTGTCCGGAGGT
GGAGCTTCCATACCCTGACCTACAGGAATTTGTAGCTGACGTCAATGTGC
TGATGGCCCTGATCATCAATGGTCCCATAAAGTCATTCTGCTACCGCCGG
CTGCAGTACCTGAGCTCCAAATTCCAGATGCACGTTTTGCTCAATGAGAT
GAAGGAGCTCGCTGCTCAGAAGAAAGTGCCACACCGGGACTTCTACAATA
TCCGTAAGGTGGACACACACATCCACGCCTCGTCCTGCATGAACCAGAAA
CATCTACTGCGCTTCATCAAGCGGGCCATGAAGCGGCACCTGGAGGAGAT
TGTGCATGTGGAACAGGGCCGCGAGCAGACGCTGAGAGAAGTCTTCGAGA
GCATGAACCTCACTGCCTACGACTTAAGTGTGGACACGCTGGATGTGCAT
GCGGACAGGAATACCTTTCATCGATTTGACAAATTCAATGCCAAATACAA
CCCTATTGGGGAGTCTGTTCTCCGAGAGATCTTCATTAAAACCGACAACA
AGATTTCTGGGAAGTACTTTGCTCACATCATCAAGGAGGTGATGGCAGAC
TTGGAGGAGAGCAAATACCAGAATGCAGAGCTCCGGCTGTCCATCTACGG
GCGTTCGAGGGATGAGTGGGACAAGCTGGCACGCTGGGCAGTGAACCACA
AAGTGCACTCTCCCAATGTCCGCTGGCTGGTGCAGGTGCCCCGCCTCTTC
GATGTGTACCGCACCAAGGGCCAGCTGGCCAACTTCCAAGAGATGCTGGA
GAACATCTTTCTGCCCCTGTTTGAGGCTACTGTGCACCCTGCCAGCCACC
CGGAGCTGCACCTCTTTCTGGAGCACGTGGATGGTTTTGATAGCGTGGAT
GATGAGTCCAAGCCAGAGAACCACGTCTTCAACCTGGAGAGTCCCCTCCC
AGAAGCTTGGGTGGAGGAGGACAACCCTCCCTATGCCTACTACCTGTACT
ACACCTTCGCTAACATGGCTATGTTGAACCATCTGCGCAGGCAGAGAGGT
TTCCACACGTTCGTGCTGAGGCCGCACTGTGGGGAGGCCGGGCCCATCCA
CCACCTGGTATCAGCCTTCATGCTGGCCGAGAACATCTCCCACGGGCTGC
TCCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTATTACCTGGCTCAGATC
GGCATCGCCATGTCCCCGCTCAGCAACAACAGCCTGTTCCTCAGCTACCA
CCGGAACCCTCTCCCTGAGTACTTGTCCCGTGGCCTCATGGTCTCGCTGT
CCACAGATGATCCCTTGCAGTTCCACTTCACCAAGGAGCCCCTGATGGAG
GAGTACAGCATCGCCACCCAGGTGTGGAAGCTCAGCTCCTGCGATATGTG
CGAGCTGGCCCGTAACAGCGTGCTCATGAGTGGCTTCTCTCACAAGGTGA
AAAGCCACTGGCTGGGACCCAACTATACCAAGGAGGGCCCTGAGGGCAAT
GATATCCGCCGTACCAACGTGCCAGACATCCGAGTGGGCTACCGCTATGA
GACGCTATGCCAGGAGCTGGCACTTATCACACAGGCCGTCCAAAGTGAGA
TGCTGGAGACCATCCCAGAGGAAGTGGGCATTGTCATGAGCCCAGGGCCT
TAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASEARSGLGASPLQSARS
LPGNAPCLKHFPLDLRTSMDGKCKEIAEELFSRSLAESELRSAPYEFPEE
SPIEQLEERRQRLERQISQDVKLEPDILLRAKQDFLKTDSDSDLQLYKEQ
GEGQGDRGLWERDVVLEREFQRVIISGEEKCGVPFTDLLDAAKSVVRALF
IREKYMALSLQSFCPTTRRYLQQLAEKPLETRTYEQSPDTPVSADAPVHP
PALEQHPYEHCEPSAMPGDLGLGLRMVRGVVHVYTRRDPDEHCPEVELPY
PDLQEFVADVNVLMALIINGPIKSFCYRRLQYLSSKFQMHVLLNEMKELA
AQKKVPHRDFYNIRKVDTHIHASSCMNQKHLLRFIKRAMKRHLEEIVHVE
QGREQTLREVFESMNLTAYDLSVDTLDVHADRNTFHRFDKFNAKYNPIGE
SVLREIFIKTDNKISGKYFAHIIKEVMADLEESKYQNAELRLSIYGRSRD
EWDKLARWAVNHKVHSPNVRWLVQVPRLFDVYRTKGQLANFQEMLENIFL
PLFEATVHPASHPELHLFLEHVDGFDSVDDESKPENHVFNLESPLPEAWV
EEDNPPYAYYLYYTFANMAMLNHLRRQRGFHTFVLRPHCGEAGPIHHLVS
AFMLAENISHGLLLRKAPVLQYLYYLAQIGIAMSPLSNNSLFLSYHRNPL
PEYLSRGLMVSLSTDDPLQFHFTKEPLMEEYSIATQVWKLSSCDMCELAR
NSVLMSGFSHKVKSHWLGPNYTKEGPEGNDIRRTNVPDIRVGYRYETLCQ
ELALITQAVQSEMLETIPEEVGIVMSPGP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12442
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 118844 Daltons