cDNA Clone EIF2AK2 (PKR)

• Plasmid: pEBG6P-PKR
• Parent Plasmid: pEBG6P
• Expressed: GST-PKR
• Genbank: NP_002750.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTGGTGATCTTTCAGCAGGTTTCTTCATGGAGGAACTTAA
TACATACCGTCAGAAGCAGGGAGTAGTACTTAAATATCAAGAACTGCCTA
ATTCAGGACCTCCACATGATAGGAGGTTTACATTTCAAGTTATAATAGAT
GGAAGAGAATTTCCAGAAGGTGAAGGTAGATCAAAGAAGGAAGCAAAAAA
TGCCGCAGCCAAATTAGCTGTTGAGATACTTAATAAGGAAAAGAAGGCAG
TTAGTCCTTTATTATTGACAACAACGAATTCTTCAGAAGGATTATCCATG
GGGAATTACATAGGCCTTATCAATAGAATTGCCCAGAAGAAAAGACTAAC
TGTAAATTATGAACAGTGTGCATCGGGGGTGCATGGGCCAGAAGGATTTC
ATTATAAATGCAAAATGGGACAGAAAGAATATAGTATTGGTACAGGTTCT
ACTAAACAGGAAGCAAAACAATTGGCCGCTAAACTTGCATATCTTCAGAT
ATTATCAGAAGAAACCTCAGTGAAATCTGACTACCTGTCCTCTGGTTCTT
TTGCTACTACGTGTGAGTCCCAAAGCAACTCTTTAGTGACCAGCACACTC
GCTTCTGAATCATCATCTGAAGGTGACTTCTCAGCAGATACATCAGAGAT
AAATTCTAACAGTGACAGTTTAAACAGTTCTTCGTTGCTTATGAATGGTC
TCAGAAATAATCAAAGGAAGGCAAAAAGATCTTTGGCACCCAGATTTGAC
CTTCCTGACATGAAAGAAACAAAGTATACTGTGGACAAGAGGTTTGGCAT
GGATTTTAAAGAAATAGAATTAATTGGCTCAGGTGGATTTGGCCAAGTTT
TCAAAGCAAAACACAGAATTGACGGAAAGACTTACGTTATTAAACGTGTT
AAATATAATAACGAGAAGGCGGAGCGTGAAGTAAAAGCATTGGCAAAACT
TGATCATGTAAATATTGTTCACTACAATGGCTGTTGGGATGGATTTGATT
ATGATCCTGAGACCAGTGATGATTCTCTTGAGAGCAGTGATTATGATCCT
GAGAACAGCAAAAATAGTTCAAGGTCAAAGACTAAGTGCCTTTTCATCCA
AATGGAATTCTGTGATAAAGGGACCTTGGAACAATGGATTGAAAAAAGAA
GAGGCGAGAAACTAGACAAAGTTTTGGCTTTGGAACTCTTTGAACAAATA
ACAAAAGGGGTGGATTATATACATTCAAAAAAATTAATTCATAGAGATCT
TAAGCCAAGTAATATATTCTTAGTAGATACAAAACAAGTAAAGATTGGAG
ACTTTGGACTTGTAACATCTCTGAAAAATGATGGAAAGCGAACAAGGAGT
AAGGGAACTTTGCGATACATGAGCCCAGAACAGATTTCTTCGCAAGACTA
TGGAAAGGAAGTGGACCTCTACGCTTTGGGGCTAATTCTTGCTGAACTTC
TTCATGTATGTGACACTGCTTTTGAAACATCAAAGTTTTTCACAGACCTA
CGGGATGGCATCATCTCAGATATATTTGATAAAAAAGAAAAAACTCTTCT
ACAGAAATTACTCTCAAAGAAACCTGAGGATCGACCTAACACATCTGAAA
TACTAAGGACCTTGACTGTGTGGAAGAAAAGCCCAGAGAAAAATGAACGA
CACACATGTTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAGDLSAGFFMEE
LNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEA
KNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKR
LTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYL
QILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTS
EINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRF
GMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALA
KLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLF
IQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHR
DLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQ
DYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKT
LLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12424
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 89510 Daltons