cDNA Clone BETA-CATENIN

• Synonyms: CTNNB1
• Plasmid: pCMV-HA-1 beta catenin
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-beta catenin
• Genbank: CAA79497
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCTACTCAAGCTGATTTGATGGAGTTGGACATGGCCATGGA
ACCAGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGG
ACTCTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGT
AAAGGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGA
GTGGGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGATA
TTGATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATG
TTCCCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGA
TGCTGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGC
TGAAACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTT
GCCACACGTGCAATCCCTGAACTGACAAAACTGCTAAATGACGAGGACCA
GGTGGTGGTTAATAAGGCTGCAGTTATGGTCCATCAGCTTTCTAAAAAGG
AAGCTTCCAGACACGCTATCATGCGTTCTCCTCAGATGGTGTCTGCTATT
GTACGTACCATGCAGAATACAAATGATGTAGAAACAGCTCGTTGTACCGC
TGGGACCTTGCATAACCTTTCCCATCATCGTGAGGGCTTACTGGCCATCT
TTAAGTCTGGAGGCATTCCTGCCCTGGTGAAAATGCTTGGTTCACCAGTG
GATTCTGTGTTGTTTTATGCCATTACAACTCTCCACAACCTTTTATTACA
TCAAGAAGGAGCTAAAATGGCAGTGCGTTTAGCTGGTGGGCTGCAGAAAA
TGGTTGCCTTGCTCAACAAAACAAATGTTAAATTCTTGGCTATTACGACA
GACTGCCTTCAAATTTTAGCTTATGGCAACCAAGAAAGCAAGCTCATCAT
ACTGGCTAGTGGTGGACCCCAAGCTTTAGTAAATATAATGAGGACCTATA
CTTACGAAAAACTACTGTGGACCACAAGCAGAGTGCTGAAGGTGCTATCT
GTCTGCTCTAGTAATAAGCCGGCTATTGTAGAAGCTGGTGGAATGCAAGC
TTTAGGACTTCACCTGACAGATCCAAGTCAACGTCTTGTTCAGAACTGTC
TTTGGACTCTCAGGAATCTTTCAGATGCTGCAACTAAACAGGAAGGGATG
GAAGGTCTCCTTGGGACTCTTGTTCAGCTTCTGGGTTCAGATGATATAAA
TGTGGTCACCTGTGCAGCTGGAATTCTTTCTAACCTCACTTGCAATAATT
ATAAGAACAAGATGATGGTCTGCCAAGTGGGTGGTATAGAGGCTCTTGTG
CGTACTGTCCTTCGGGCTGGTGACAGGGAAGACATCACTGAGCCTGCCAT
CTGTGCTCTTCGTCATCTGACCAGCCGACACCAAGAAGCAGAGATGGCCC
AGAATGCAGTTCGCCTTCACTATGGACTACCAGTTGTGGTTAAGCTCTTA
CACCCACCATCCCACTGGCCTCTGATAAAGGCTACTGTTGGATTGATTCG
AAATCTTGCCCTTTGTCCCGCAAATCATGCACCTTTGCGTGAGCAGGGTG
CCATTCCACGACTAGTTCAGTTGCTTGTTCGTGCACATCAGGATACCCAG
CGCCGTACGTCCATGGGTGGGACACAGCAGCAATTTGTGGAGGGGGTCCG
CATGGAAGAAATAGTTGAAGGTTGTACCGGAGCCCTTCACATCCTAGCTC
GGGATGTTCACAACCGAATTGTTATCAGAGGACTAAATACCATTCCATTG
TTTGTGCAGCTGCTTTATTCTCCCATTGAAAACATCCAAAGAGTAGCTGC
AGGGGTCCTCTGTGAACTTGCTCAGGACAAGGAAGCTGCAGAAGCTATGG
AAGCTGAGGGAGCCACAGCTCCTCTGACAGAGTTACTTCACTCTAGGAAT
GAAGGTGTGGCGACATATGCAGCTGCTGTTTTGTTCCGAATGTCTGAGGA
CAAGCCACAAGATTACAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTC
TCTTCAGAACAGAGCCAATGGCTTGGAATGAGACTGCTGATCTTGGACTT
GATATTGGTGCCCAGGGAGAACCCCTTGGATATCGCCAGGATGATCCTAG
CTATCGTTCTTTTCACTCTGGTGGATATGGCCAGGATGCCTTGGGTATGG
ACCCCATGATGGAACATGAGATGGGTGGCCACCACCCTGGTGCTGACTAT
CCAGTTGATGGGCTGCCAGATCTGGGGCATGCCCAGGACCTCATGGATGG
GCTGCCTCCAGGTGACAGCAATCAGCTGGCCTGGTTTGATACTGACCTGT
AAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSG
ATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAM
TRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVN
LINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAI
MRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIP
ALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNK
TNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLW
TTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNL
SDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMV
CQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLH
YGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQ
LLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRI
VIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAMEAEGATA
PLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPM
AWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHE
MGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12422
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression. As genbank but has I631M which was in original template.; ; Estimated MW of expressed protein : 86869 Daltons