cDNA Clone UBE2T

• Plasmid: pET15b6P UBE2T
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2T
• Genbank: NP_054895.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGAGAGCTTCACGTCTGAAGAGAGAGCTGCACATGTTAGC
CACAGAGCCACCCCCAGGCATCACATGTTGGCAAGATAAAGACCAAATGG
ATGACCTGCGAGCTCAAATATTAGGTGGAGCCAACACACCTTATGAGAAA
GGTGTTTTTAAGCTAGAAGTTATCATTCCTGAGAGGTACCCATTTGAACC
TCCTCAGATCCGATTTCTCACTCCAATTTATCATCCAAACATTGATTCTG
CTGGAAGGATTTGTCTGGATGTTCTCAAATTGCCACCAAAAGGTGCTTGG
AGACCATCCCTCAACATCGCAACTGTGTTGACCTCTATTCAGCTGCTCAT
GTCAGAACCCAACCCTGATGACCCGCTCATGGCTGACATATCCTCAGAAT
TTAAATATAATAAGCCAGCCTTCCTCAAGAATGCCAGACAGTGGACAGAG
AAGCATGCAAGACAGAAACAAAAGGCTGATGAGGAAGAGATGCTTGATAA
TCTACCAGAGGCTGGTGACTCCAGAGTACACAACTCAACACAGAAAAGGA
AGGCCAGTCAGCTAGTAGGCATAGAAAAGAAATTTCATCCTGATGTTTAG
GCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMQRASRLKRELHMLATEPPPGITCWQD
KDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHP
NIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMAD
ISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNS
TQKRKASQLVGIEKKFHPDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12416
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 24964 Daltons