cDNA Clone BETA-CATENIN

• Synonyms: CTNNB1
• Plasmid: pEBG6P-Beta Catenin
• Parent Plasmid: pEBG6P
• Expressed: GST-Beta Catenin
• Genbank: CAA79497
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTACTCAAGCTGATTTGATGGAGTTGGACATGGCCATGGA
ACCAGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGG
ACTCTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGT
AAAGGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGA
GTGGGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGATA
TTGATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATG
TTCCCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGA
TGCTGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGC
TGAAACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTT
GCCACACGTGCAATCCCTGAACTGACAAAACTGCTAAATGACGAGGACCA
GGTGGTGGTTAATAAGGCTGCAGTTATGGTCCATCAGCTTTCTAAAAAGG
AAGCTTCCAGACACGCTATCATGCGTTCTCCTCAGATGGTGTCTGCTATT
GTACGTACCATGCAGAATACAAATGATGTAGAAACAGCTCGTTGTACCGC
TGGGACCTTGCATAACCTTTCCCATCATCGTGAGGGCTTACTGGCCATCT
TTAAGTCTGGAGGCATTCCTGCCCTGGTGAAAATGCTTGGTTCACCAGTG
GATTCTGTGTTGTTTTATGCCATTACAACTCTCCACAACCTTTTATTACA
TCAAGAAGGAGCTAAAATGGCAGTGCGTTTAGCTGGTGGGCTGCAGAAAA
TGGTTGCCTTGCTCAACAAAACAAATGTTAAATTCTTGGCTATTACGACA
GACTGCCTTCAAATTTTAGCTTATGGCAACCAAGAAAGCAAGCTCATCAT
ACTGGCTAGTGGTGGACCCCAAGCTTTAGTAAATATAATGAGGACCTATA
CTTACGAAAAACTACTGTGGACCACAAGCAGAGTGCTGAAGGTGCTATCT
GTCTGCTCTAGTAATAAGCCGGCTATTGTAGAAGCTGGTGGAATGCAAGC
TTTAGGACTTCACCTGACAGATCCAAGTCAACGTCTTGTTCAGAACTGTC
TTTGGACTCTCAGGAATCTTTCAGATGCTGCAACTAAACAGGAAGGGATG
GAAGGTCTCCTTGGGACTCTTGTTCAGCTTCTGGGTTCAGATGATATAAA
TGTGGTCACCTGTGCAGCTGGAATTCTTTCTAACCTCACTTGCAATAATT
ATAAGAACAAGATGATGGTCTGCCAAGTGGGTGGTATAGAGGCTCTTGTG
CGTACTGTCCTTCGGGCTGGTGACAGGGAAGACATCACTGAGCCTGCCAT
CTGTGCTCTTCGTCATCTGACCAGCCGACACCAAGAAGCAGAGATGGCCC
AGAATGCAGTTCGCCTTCACTATGGACTACCAGTTGTGGTTAAGCTCTTA
CACCCACCATCCCACTGGCCTCTGATAAAGGCTACTGTTGGATTGATTCG
AAATCTTGCCCTTTGTCCCGCAAATCATGCACCTTTGCGTGAGCAGGGTG
CCATTCCACGACTAGTTCAGTTGCTTGTTCGTGCACATCAGGATACCCAG
CGCCGTACGTCCATGGGTGGGACACAGCAGCAATTTGTGGAGGGGGTCCG
CATGGAAGAAATAGTTGAAGGTTGTACCGGAGCCCTTCACATCCTAGCTC
GGGATGTTCACAACCGAATTGTTATCAGAGGACTAAATACCATTCCATTG
TTTGTGCAGCTGCTTTATTCTCCCATTGAAAACATCCAAAGAGTAGCTGC
AGGGGTCCTCTGTGAACTTGCTCAGGACAAGGAAGCTGCAGAAGCTATGG
AAGCTGAGGGAGCCACAGCTCCTCTGACAGAGTTACTTCACTCTAGGAAT
GAAGGTGTGGCGACATATGCAGCTGCTGTTTTGTTCCGAATGTCTGAGGA
CAAGCCACAAGATTACAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTC
TCTTCAGAACAGAGCCAATGGCTTGGAATGAGACTGCTGATCTTGGACTT
GATATTGGTGCCCAGGGAGAACCCCTTGGATATCGCCAGGATGATCCTAG
CTATCGTTCTTTTCACTCTGGTGGATATGGCCAGGATGCCTTGGGTATGG
ACCCCATGATGGAACATGAGATGGGTGGCCACCACCCTGGTGCTGACTAT
CCAGTTGATGGGCTGCCAGATCTGGGGCATGCCCAGGACCTCATGGATGG
GCTGCCTCCAGGTGACAGCAATCAGCTGGCCTGGTTTGATACTGACCTGT
AAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMATQADLMELDMA
MEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVL
YEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQ
FDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARC
TAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLL
LHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKL
IILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGM
QALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDD
INVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEP
AICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGL
IRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEG
VRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRV
AAGVLCELAQDKEAAEAMEAEGATAPLTELLHSRNEGVATYAAAVLFRMS
EDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDD
PSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLM
DGLPPGDSNQLAWFDTDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12408
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression. As genbank but has I631M which was in original template.; ; Estimated MW of expressed protein : 112927 Daltons