cDNA Clone Optineurin

• Plasmid: pCMV5 Optineurin HA E50K
• Parent Plasmid: pCMV5
• Expressed: Optineurin-HA E50K
• Genbank: NP_001008212
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGA
GGACAGCCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACC
CAAACCTGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAG
CTCCTGACCAAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCA
AGCCATGAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGA
AGGAAGAACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGT
CTAATGGCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAA
ACTAAAAGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCA
GGCTTCCCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAG
GTGGTGAGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGA
ACTGCAGCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTG
AAATTAGGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAG
CATAGTCCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAA
ATATAGGAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGG
AGTTAACTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAG
GTGGAGAGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGA
TTTTGAAAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGG
GGAGCACAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGA
AGCGAAGTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCT
TCAAGAGGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGAC
TTCAAGAAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCA
GAGTTGAATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCT
ACAAGTGGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAG
AGGATGAAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTT
CTTCAAGAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAA
AGAGTCAGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAAC
TGGAACTGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAA
ATGAAGCAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCAT
CCTCAGGGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAG
CAGCGAGAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTG
GCAGTTCTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTC
CTTGATGGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACC
AGCAGGCTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAA
CAGCGGAATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCC
TGACATAGACACGTTACAGATTCACGTGATGGATTGCATCATTATGTACC
CATACGATGTGCCAGATTACGCCTAAGGATCC

Amino Acid Sequence

MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTK
NHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMAL
SHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRL
QAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPG
PTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERL
EVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVE
ALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNE
KQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEH
NNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQT
IAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLL
KENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNI
PIHSCPKCGEVLPDIDTLQIHVMDCIIMYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12371
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 67134 Daltons