cDNA Clone DYRK1B p69

• Plasmid: pFBGST DYRK1b isoform a
• Parent Plasmid: pFastbac GST
• Expressed: GST DYRK1B isoform a
• Genbank: NM_004714.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACCATGGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACGCAGGTATTGCCTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTCCGGGATGCAACCTCAGCCCCGCTGCGTAAGCTCTCTGTGGACCTCATCAAGACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGGGCCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGTCCTGAACCATGGTTATGATGACGACAACCATGACTACATCGTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTGGCCATCAAGATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTGATGAACCAGCATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTGCCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACACCCACTTCCGCGGCGTCTCGCTGAACCTGACCCGGAAGCTGGCGCAGCAGCTCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCAGCATCATTCACTGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCGCCATCAAGATTGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGCCGCTTCTACCGCTCACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTTGTGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGACCAGATGAACCGCATTGTGGAGGTGCTGGGCATCCCACCGGCCGCCATGCTGGACCAGGCGCCCAAGGCTCGCAAGTACTTTGAACGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGATTACCAGGGCCCCGGGACACGGCGGCTGCAGGAGGTGCTGGGCGTGCAGACGGGCGGGCCCGGGGGCCGGCGGGCGGGGGAGCCGGGCCACAGCCCCGCCGACTACCTCCGCTTCCAGGACCTGGTGCTGCGCATGCTGGAGTATGAGCCCGCCGCCCGCATCAGCCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCTTCCAGCACCGCCAGCTCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTCCAGTGACAACCGGACCTACCGCTACAGCAACCGATATTGTGGGGGCCCTGGGCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGTCCCACCCTCCCAGCCACTGCGGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCAAGCCCCTGCCTCTGCCTCGTCACTGCCTGGGACCGGGGCCCAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCACCACCACCCCCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCCACCTCACCCAGCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGTCCACCCCTCCCGCCTCCTGATGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGAGCACAGCAGCCAGCTCGTGACCCTGCCCCCTCCCTGGGGCCCCTCCTGAAGCCATAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTMAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKK
RRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTE
MKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQ
RIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQ
GPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSIS
SSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSL
VGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12362
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression.; ; Estimated MW of expressed protein : 91755 Daltons
• Low Temp: No