cDNA Clone IRAK1

• Plasmid: pHR-SIN-CSGW FLAG IRAK1 Y288g
• Parent Plasmid: pHR SIN CSGW
• Expressed: FLAG IRAK1 Y288g
• Genbank: AAC41949
• Tag: FLAG
• Species: Human

Sequence of Insert

ACTAGTGCCACCATGGACTACAAGGACGATGACGATAAGATGGCCGGGGG
GCCGGGCCCGGGGGAGCCCGCAGCCCCCGGCGCCCAGCACTTCTTGTACG
AGGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTG
GAGCCCGCCGACTGGTGCCAGTTCGCCGCCCTGATCGTGCGCGACCAGAC
CGAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGT
GGCCCTGGATCAACCGCAACGCCCGTGTGGCCGACCTCGTGCACATCCTC
ACGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCC
TCCCGCCCCGCTTCCGTCCCCAGGCACCACTGCCCCGAGGCCCAGCAGCA
TCCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCC
TCAGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTC
AGGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCAC
CGCCATCTCCAGCCCCTTCTTCTACCAAGCCAGGCCCAGAGAGCTCAGTG
TCCCTCCTGCAGGGAGCCCGCCCCTCTCCGTTTTGCTGGCCCCTCTGTGA
GATTTCCCGGGGCACCCACAACTTCTCGGAGGAGCTCAAGATCGGGGAGG
GTGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCT
GTGAAGAGGCTGAAGGAGAACGCTGACCTGGAGTGGACTGCAGTGAAGCA
GAGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACA
TTGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTG
GGCGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGAC
CCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGG
GTACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATC
CATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACC
CAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCA
GCCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACC
CTGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGA
CACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTC
AGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTG
GTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAG
CACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCA
TGCAGATCTACAAGAAGCACCTGGACCCCAGGCCCGGGCCCTGCCCACCT
GAGCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCG
GGCCAAAAGGAGGCCTCCTATGACCCAGGTGTACGAGAGGCTAGAGAAGC
TGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTTGGAGGCCGCCAGCTGC
ATCCCCCCTTCCCCGCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGC
CCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCA
GTGCCCAGGCAGCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAG
AGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTT
GACTCCAAGCTGCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTC
CTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCC
CGGCCCACAGCCGTGGAAGGACTGGCCCTTGGCAGCTCTGCATCATCGTC
GTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCC
AGAAGCTGGCCCTGTACGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTG
TCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCC
CGAAGAAAGTGATGAATTTCAGAGCTGACATATG

Amino Acid Sequence

MDYKDDDDKMAGGPGPGEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPAD
WCQFAALIVRDQTELRLCERSGQRTASVLWPWINRNARVADLVHILTHLQ
LLRARDIITAWHPPAPLPSPGTTAPRPSSIPAPAEAEAWSPRKLPSSAST
FLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPSSTKPGPESSVSLLQ
GARPSPFCWPLCEISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVKRL
KENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVGGFL
PNGSLEDRLHCQTQACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDI
KSSNVLLDERLTPKLGDFGLARFSRFAGSSPSQSSMVARTQTVRGTLAYL
PEEYIKTGRLAVDTDTFSFGVVVLETLAGQRAVKTHGARTKYLKDLVEEE
AEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPELGL
GLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAGVPGHLEAASCIPPS
PQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDES
LGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTA
VEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSS
LPGLGLEQDRQGPEESDEFQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12359
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for selectable expression (Puro) in mammalian cells.; ; Estimated MW of expressed protein : 77518 Daltons