cDNA Clone PPP4R3B

• Plasmid: pCMV-HA-3 PPP4R3B
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-PPP4R3B
• Genbank: NP_065196.1
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCGGCCGCGATGTCGGATACGCGGCGGCGAGTGAAGGTCTATAC
CCTGAACGAAGACCGGCAATGGGACGACCGAGGCACCGGGCACGTCTCCT
CCACTTACGTGGAGGAGCTCAAGGGGATGTCGCTGCTGGTTCGGGCAGAG
TCCGACGGATCACTACTCTTGGAATCAAAGATAAATCCAAATACTGCATA
TCAGAAACAACAGGATACATTAATTGTTTGGTCAGAAGCAGAGAACTATG
ATTTGGCTCTGAGTTTTCAGGAGAAAGCTGGCTGTGATGAGATCTGGGAA
AAAATTTGTCAGGTTCAAGGTAAAGACCCATCAGTGGAAGTCACACAGGA
CCTCATTGATGAATCTGAAGAAGAACGATTTGAAGAAATGCCTGAAACTA
GTCATCTGATTGACCTGCCCACATGTGAACTCAATAAACTTGAAGAGATT
GCTGACTTAGTTACCTCAGTGCTCTCCTCACCTATCCGTAGGGAAAAGCT
GGCTCTCGCCTTGGAAAATGAAGGCTATATTAAAAAACTATTGCAGCTGT
TCCAAGCTTGCGAGAACCTAGAAAACACTGAAGGCTTACACCATTTGTAT
GAAATTATTAGAGGAATCTTATTCCTAAATAAGGCAACTCTTTTTGAGGT
AATGTTTTCTGATGAGTGTATCATGGATGTCGTGGGATGCCTTGAATATG
ACCCTGCTTTGGCTCAGCCAAAAAGACATAGAGAATTCTTGACCAAAACT
GCAAAGTTCAAGGAAGTTATACCAATAACAGACTCTGAACTAAGGCAAAA
AATACATCAGACTTACAGGGTACAGTACATTCAGGACATCATTTTGCCCA
CACCATCTGTTTTTGAAGAGAATTTTCTTTCTACTCTTACGTCTTTTATT
TTCTTCAACAAAGTTGAGATAGTCAGCATGTTGCAGGAAGATGAGAAGTT
TTTGTCTGAAGTTTTTGCACAATTAACAGATGAGGCTACAGATGATGATA
AACGGCGTGAATTGGTTAATTTTTTCAAGGAGTTTTGTGCATTTTCTCAG
ACATTACAACCTCAAAACAGGGATGCATTTTTCAAAACATTGGCAAAATT
GGGAATTCTTCCTGCTCTTGAAATTGTAATGGGCATGGATGATTTGCAAG
TCAGATCAGCTGCTACAGATATATTTTCTTATCTAGTAGAATTTAGTCCA
TCTATGGTCCGAGAGTTTGTAATGCAAGAAGCTCAGCAGAGTGATGACGA
TATTCTTCTTATTAATGTGGTAATTGAACAAATGATCTGTGATACTGATC
CTGAGCTAGGAGGCGCTGTTCAGTTAATGGGACTTCTTCGTACTCTAATT
GATCCAGAGAACATGCTGGCTACAACTAATAAAACTGAAAAAAGTGAATT
TCTAAATTTTTTCTACAACCATTGTATGCATGTTCTCACAGCACCACTTT
TGACCAATACTTCAGAAGACAAATGTGAAAAGGATAATATAGTTGGATCA
AACAAAAACAACACAATTTGTCCCGGTGCCCTTCGCTTTATGAGGCGGAT
AATTGGACTTAAAGATGAATTTTATAATCGTTACATCACCAAGGGAAATC
TTTTTGAGCCAGTTATAAATGCACTTCTGGATAATGGAACTCGGTATAAT
CTGTTGAATTCAGCTGTTATTGAGTTGTTTGAATTTATAAGAGTGGAAGA
TATCAAGTCTCTTACTGCCCATATAGTTGAAAACTTTTATAAAGCACTTG
AATCGATTGAATATGTTCAGACATTCAAAGGATTGAAGACTAAATATGAG
CAAGAAAAAGACAGACAAAATCAGAAACTGAACAGTGTACCATCTATATT
GCGTAGTAACAGATTTCGCAGAGATGCAAAAGCCTTGGAAGAGGATGAAG
AAATGTGGTTTAATGAAGATGAAGAAGAGGAAGGAAAAGCAGTTGTGGCA
CCAGTGGAAAAACCTAAGCCAGAAGATGATTTTCCAGATAATTATGAAAA
GTTTATGGAGACTAAAAAAGCAAAAGAAAGTGAAGACAAGGAAAACCTTC
CCAAAAGGACATCTCCTGGTGGCTTCAAATTTACTTTCTCCCACTCTGCC
AGTGCTGCTAATGGAACAAACAGTAAATCTGTAGTGGCTCAGATACCACC
AGCAACTTCTAATGGATCCTCTTCCAAAACCACAAACTTGCCTACGTCAG
TAACAGCCACCAAGGGAAGTTTGGTTGGCTTAGTGGATTATCCAGATGAT
GAAGAGGAAGATGAAGAAGAAGAATCGTCCCCCAGGAAAAGACCTCGTCT
TGGCTCATAAGCGGCCGCGTCGAC

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDAAAMSDTRRRVKVYTLNEDRQWDDRGTGHVS
STYVEELKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENY
DLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDLIDESEEERFEEMPET
SHLIDLPTCELNKLEEIADLVTSVLSSPIRREKLALALENEGYIKKLLQL
FQACENLENTEGLHHLYEIIRGILFLNKATLFEVMFSDECIMDVVGCLEY
DPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILP
TPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDD
KRRELVNFFKEFCAFSQTLQPQNRDAFFKTLAKLGILPALEIVMGMDDLQ
VRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVIEQMICDTD
PELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPL
LTNTSEDKCEKDNIVGSNKNNTICPGALRFMRRIIGLKDEFYNRYITKGN
LFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKAL
ESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDE
EMWFNEDEEEEGKAVVAPVEKPKPEDDFPDNYEKFMETKKAKESEDKENL
PKRTSPGGFKFTFSHSASAANGTNSKSVVAQIPPATSNGSSSKTTNLPTS
VTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12356
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 89799 Daltons