cDNA Clone AUK1

• Plasmid: pGEX6P-1-tb AUK1
• Parent Plasmid: pGEX6P1
• Genbank: XP_828896.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCATGAGGTCAACTGAGGTCGGGCGTGTTGTGGAGGATTTTATTGT
ACTCCCTCCGACACCCAAATCCAAGTGGAAGTTGTCTGATTTCGAGCTTT
TGCACAAACTTGGCGGCGGCAATTACGGGGATGTACACCTCGCCTCCGTG
AAGGATTGCAATTTTGTTTGCGCGCTGAAAAGGTTGTCCATTAAAAAACT
TGCGGATTTTGACATTGCGACGCAACTCCGTCGTGAAATTGAGATCGCTT
TTAACACGCGTCACAAGTACCTGCTGCGAACCTATGCATACTTCTTTGAC
GAAACTGATATTTATCTCATTATGGAGCCATGCAGCAACGGCATGCTTTA
CACGGAACTCAACCGAGTGAAATGCTTTGCGCCACCGACAGCAGCCCGTT
ACGTGGCTCAGCTCGCTGAAGCCCTCCTCTACTTGCATCAACATCACATC
CTCCACCGCGACATTAAGCCGGAGAATATTTTACTAGATCACAACAATAA
CATCAAACTGGCGGATTTCGGTTGGTCAGTCCACGATCCGGATAACCGTC
GCAAGACATCTTGCGGGACGCCAGAGTATTTTCCACCAGAAATTGTGGGA
CGACAAGCGTACGATACGAGTGCTGACCTTTGGTGCCTGGGCATCTTCTG
TTATGAGCTTCTCGTTGGTAAAACACCGTTTGTTGGAAAGGATACAGACC
AAATCTGCAAGAACATTCATTCCATGCACTTCAAAATACCGGAGAATATA
CCGTCAGAAGCGAAGGACCTCATCGCGAACTTGCTTCTGCGTGATGGGTC
ACGGAGGCTTGCACTTCACCGTGTCGTGAATCACCAGTTCCTTCTCAAGT
ATTATTATCTCCCAAACAATTTACAACCTCCCACTGGAAAGCGTCCGCGT
CTCGATGCAGAGCCAACTGCAGGGAAAGAGAATTAAGCGGCCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMRSTEVGRVVEDFIVLPPT
PKSKWKLSDFELLHKLGGGNYGDVHLASVKDCNFVCALKRLSIKKLADFD
IATQLRREIEIAFNTRHKYLLRTYAYFFDETDIYLIMEPCSNGMLYTELN
RVKCFAPPTAARYVAQLAEALLYLHQHHILHRDIKPENILLDHNNNIKLA
DFGWSVHDPDNRRKTSCGTPEYFPPEIVGRQAYDTSADLWCLGIFCYELL
VGKTPFVGKDTDQICKNIHSMHFKIPENIPSEAKDLIANLLLRDGSRRLA
LHRVVNHQFLLKYYYLPNNLQPPTGKRPRLDAEPTAGKEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12333
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 62377 Daltons