cDNA Clone IRAK1

• Plasmid: pCMV-FLAG-2 IRAK1 K239A
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG IRAK1 K239A
• Genbank: pCMV FLAG-2
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGCAGCCCCCGG
CGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCT
ACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCCGCC
CTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCA
GCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACGCCCGTGTGG
CCGACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGAC
ATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCCAGGCACCAC
TGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGA
GCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCCCCAGCTTTT
CCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCC
TGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTTCTACCAAGC
CAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTCTCCG
TTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGA
GGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGA
TGAGGAACACGGTGTATGCTGTGGCGAGGCTGAAGGAGAACGCTGACCTG
GAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTC
CAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGA
ACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAG
GACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCA
GCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATC
AGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTT
CTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTT
CAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGA
CACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAG
ACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGT
GCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGA
CCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTG
GCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGC
CTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGACCCCA
GGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCC
TGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGT
GTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGC
ATTTGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTAC
GTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCT
GGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAG
GCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCT
GCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACC
CCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCT
GGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTT
GGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCC
TGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCC
TGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTG
GAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGAGC
GGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMAGGPGPGEPAAPGAQHFLYEVPPWVMC
RFYKVMDALEPADWCQFAALIVRDQTELRLCERSGQRTASVLWPWINRNA
RVADLVHILTHLQLLRARDIITAWHPPAPLPSPGTTAPRPSSIPAPAEAE
AWSPRKLPSSASTFLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPSS
TKPGPESSVSLLQGARPSPFCWPLCEISRGTHNFSEELKIGEGGFGCVYR
AVMRNTVYAVARLKENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYC
AQNGFYCLVYGFLPNGSLEDRLHCQTQACPPLSWPQRLDILLGTARAIQF
LHQDSPSLIHGDIKSSNVLLDERLTPKLGDFGLARFSRFAGSSPSQSSMV
ARTQTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVVLETLAGQRAVKTHG
ARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHL
DPRPGPCPPELGLGLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAGV
PGHLEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQL
QRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGE
SSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYED
GALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12312
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG tag for mammalian expression (kinase dead); ; Estimated MW of expressed protein : 78716 Daltons