cDNA Clone CYC6

• Plasmid: pFastbacDual 6P1 CYC6
• Parent Plasmid: pFastbacDual 6P1
• Genbank: XP_829729.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCATGAATCCCACGGCACTTCGTGAGGTCTCAAACCTGTCCACAGG
TTCTGGAGATGTGAAGCAGCGACTTGCTCAGCGGCGCAGCGCTAATTTAG
AGTCATGTCGTTATCCTTACTCATCCAGCGCTGTGCGACACGGCTCCGGC
AGTTTGTTAAGCCTTCGCTCAAGTCTCTACAGCCCCGTAGGTACGGCAGG
GAAGGAGTCATCTGTAAGCCCTCATGCTGACCGGCTTCGTGAGAATAATC
CGGTATACTGCAGCGATGTTATTAAGGACATCACAACAATGTACATGGAA
AGTGAGAAGGAGGCTATATACGGTCAGGTACTTCCGTCACCCCGCTATTT
AACGTACCAGCCAGAGATAAACGAGAAAATGCGAATGATTCTCGTCGATT
GGCTTATTGACGTTCACCTAAAGTTTAAACTACATCCCGAGACGCTTTAC
CTCACGGTGAGCATCGTAGACCGCTACCTTTCTTCTGTTAACACGAGGCG
TACGACCGGACGGTACATACCGCGTTCAAAGCTGCAGTTAGTTGGTATCA
CCGCCATTCTTCTTGCTGCCAAGTACGAAGAGATATGGCCTCCGGAGGTG
AAGGAGTGCGTGTACATATGTGCCAATACCTACACTCGTGATGAGGTTAT
TCGCATGGAACGTGAGATGTGCACCGAGCTGTCCTTCCGCTTTACTGTTC
CAACGCCGTTTCCTTTCATTGTTCGATTACTTGACGTTGTTGAGGGCCTC
GAGCGGCAGCAGCAGACGCAACAGTTGTCACATCAGCTACAGCAGATCCA
ATCGCAGTCGCAATCTCAATCTCAACAGACACAAATGCAAGCATCACAGT
CACAACCACAATCTCTGTCGCGTTCCCGCTCACAGTCGCGTTCGCAGCCG
CGCTCTGATGAGGAATGCACGGCATATGTTCTACAGCTTCGGCACACCGC
TTTCTTTTTTCTTGACCACGGCATGCTTGATTACAAATGCCTTCAGTTCT
CCCCGTCGCAGCAAGCAAAGGCCGCCTTGTTTCTTGCACTAGTGACACTA
CACATGAAAGAGCAAGGCTCATCGTATGTGCTGAACAACGATATCATATG
GACTCGTCAGCTTCAGTACTACAGTAAGGCTCAAGTACGTGATTTCAAGG
CGTGCGCGAGGACCATGTTAGATTTTGTGAGTTACGTCTCCACAACGCGT
TACCAATCGGTGCGTCGCAAGTACAGCAGCTCAAAGTATGGTGAAGTGGC
TAAACTGACTATGCCTAGTGAAGTACCTGACTTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNPTALREVSNLSTGSGDV
KQRLAQRRSANLESCRYPYSSSAVRHGSGSLLSLRSSLYSPVGTAGKESS
VSPHADRLRENNPVYCSDVIKDITTMYMESEKEAIYGQVLPSPRYLTYQP
EINEKMRMILVDWLIDVHLKFKLHPETLYLTVSIVDRYLSSVNTRRTTGR
YIPRSKLQLVGITAILLAAKYEEIWPPEVKECVYICANTYTRDEVIRMER
EMCTELSFRFTVPTPFPFIVRLLDVVEGLERQQQTQQLSHQLQQIQSQSQ
SQSQQTQMQASQSQPQSLSRSRSQSRSQPRSDEECTAYVLQLRHTAFFFL
DHGMLDYKCLQFSPSQQAKAALFLALVTLHMKEQGSSYVLNNDIIWTRQL
QYYSKAQVRDFKACARTMLDFVSYVSTTRYQSVRRKYSSSKYGEVAKLTM
PSEVPDF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression.; ; Estimated MW of expressed protein : 75787 Daltons