cDNA Clone CIKS

• Plasmid: pCMV-HA-1 CIKS
• Parent Plasmid: pCMV5 HA1
• Genbank: NP_671733.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCTCCTCAGCTTCAAGAAACTAGAATGAACCGAAGCATTCC
TGTGGAGGTTGATGAATCAGAACCATACCCAAGTCAGTTGCTGAAACCAA
TCCCAGAATATTCCCCGGAAGAGGAATCAGAACCACCTGCTCCAAATATA
AGGAACATGGCACCCAACAGCTTGTCTGCACCCACAATGCTTCACAATTC
CTCCGGAGACTTTTCTCAAGCTCACTCAACCCTGAAACTTGCAAATCACC
AGCGGCCTGTATCCCGGCAGGTCACCTGCCTGCGCACTCAAGTTCTGGAG
GACAGTGAAGACAGTTTCTGCAGGAGACACCCAGGCCTGGGCAAAGCTTT
CCCTTCTGGGTGCTCTGCAGTCAGCGAGCCTGCGTCTGAGTCTGTGGTTG
GAGCCCTCCCTGCAGAGCATCAGTTTTCATTTATGGAAAAACGTAATCAA
TGGCTGGTATCTCAGCTTTCAGCGGCTTCTCCTGACACTGGCCATGACTC
AGACAAATCAGACCAAAGTTTACCTAATGCCTCAGCAGACTCCTTGGGCG
GTAGCCAGGAGATGGTGCAACGGCCCCAGCCTCACAGGAACCGAGCAGGC
CTGGATCTGCCAACCATAGACACGGGATATGATTCCCAGCCCCAGGATGT
CCTGGGCATCAGGCAGCTGGAAAGGCCCCTGCCCCTCACCTCCGTGTGTT
ACCCCCAGGACCTCCCCAGACCTCTCAGGTCCAGGGAGTTCCCTCAGTTT
GAACCTCAGAGGTATCCAGCATGTGCACAGATGCTGCCTCCCAATCTTTC
CCCACATGCTCCATGGAACTATCATTACCATTGTCCTGGAAGTCCCGATC
ACCAGGTGCCATATGGCCATGACTACCCTCGAGCAGCCTACCAGCAAGTG
ATCCAGCCGGCTCTGCCTGGGCAGCCCCTGCCTGGAGCCAGTGTGAGAGG
CCTGCACCCTGTGCAGAAGGTTATCCTGAATTATCCCAGCCCCTGGGACC
AAGAAGAGAGGCCCGCACAGAGAGACTGCTCCTTTCCGGGGCTTCCAAGG
CACCAGGACCAGCCACATCACCAGCCACCTAATAGAGCTGGTGCTCCTGG
GGAGTCCTTGGAGTGCCCTGCAGAGCTGAGACCACAGGTTCCCCAGCCTC
CGTCCCCAGCTGCTGTGCCTAGACCCCCTAGCAACCCTCCAGCCAGAGGA
ACTCTAAAAACAAGCAATTTGCCAGAAGAATTGCGGAAAGTCTTTATCAC
TTATTCGATGGACACAGCTATGGAGGTGGTGAAATTCGTGAACTTTTTGT
TGGTAAATGGCTTCCAAACTGCAATTGACATATTTGAGGATAGAATCCGA
GGCATTGATATCATTAAATGGATGGAGCGCTACCTTAGGGATAAGACCGT
GATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGACGTGGAAGGCG
CTGAGTCGCAGCTGGACGAGGATGAGCATGGCTTACATACTAAGTACATT
CATCGAATGATGCAGATTGAGTTCATAAAACAAGGAAGCATGAATTTCAG
ATTCATCCCTGTGCTCTTCCCAAATGCTAAGAAGGAGCATGTGCCCACCT
GGCTTCAGAACACTCATGTCTACAGCTGGCCCAAGAATAAAAAAAACATC
CTGCTGCGGCTGCTGAGAGAGGAAGAGTATGTGGCTCCTCCACGGGGGCC
TCTGCCCACCCTTCAGGTGGTTCCCTTGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMPPQLQETRMNRSIPVEVDESEPYPSQLLKPIPEYSPE
EESEPPAPNIRNMAPNSLSAPTMLHNSSGDFSQAHSTLKLANHQRPVSRQ
VTCLRTQVLEDSEDSFCRRHPGLGKAFPSGCSAVSEPASESVVGALPAEH
QFSFMEKRNQWLVSQLSAASPDTGHDSDKSDQSLPNASADSLGGSQEMVQ
RPQPHRNRAGLDLPTIDTGYDSQPQDVLGIRQLERPLPLTSVCYPQDLPR
PLRSREFPQFEPQRYPACAQMLPPNLSPHAPWNYHYHCPGSPDHQVPYGH
DYPRAAYQQVIQPALPGQPLPGASVRGLHPVQKVILNYPSPWDQEERPAQ
RDCSFPGLPRHQDQPHHQPPNRAGAPGESLECPAELRPQVPQPPSPAAVP
RPPSNPPARGTLKTSNLPEELRKVFITYSMDTAMEVVKFVNFLLVNGFQT
AIDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDE
DEHGLHTKYIHRMMQIEFIKQGSMNFRFIPVLFPNAKKEHVPTWLQNTHV
YSWPKNKKNILLRLLREEEYVAPPRGPLPTLQVVPL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12262
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 66014 Daltons