cDNA Clone IKK alpha

• Plasmid: pAS2.6 IKK alpha
• Parent Plasmid: pAS2.6
• Expressed: GAL4 DNA BD-IKK alpha
• Genbank: O15111
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC
CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGTCTGTC
TGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCGC
CTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGAT
TATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCTG
AAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATAC
TGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTG
TGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTG
GGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAAAACCT
GAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAAT
TGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTT
TTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCT
TACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATG
TATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGC
ATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAG
ATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAATAGCCT
TTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTGA
ATTGCGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAG
CCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACA
CATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACCACCTG
ATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATA
AATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCC
TCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGATA
GCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCA
TTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAG
CAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGAAGCAG
TGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTCAGGGA
CAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAA
AATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGG
AGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAG
ATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAAT
GGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGG
AGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGAAGAGC
CCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAGCGTGC
CATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACA
GTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAG
GACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTG
TAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGTA
ATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAAGGCAG
AAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCTGCCCG
GTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCAG
CATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTG
GTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTT
GAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGAGGAAC
AGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAATGAGCG
GCCGC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGSPEFGRRAAAMERPPGLRPGAGGPWEMRERLGTGGFGNVCL
YQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPE
ELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSG
IRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSF
VGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWH
EKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLIVEPMENWLQLMLN
CDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPD
ESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDS
YMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAV
HYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLE
FFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLE
DQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYS
DSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSN
IKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSA
WLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQ
GNSMMNLDWSWLTE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU12244
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Y2H construct.; ; Estimated MW of expressed protein : 103769 Daltons