cDNA Clone MAP4K3

• Synonyms: GLK, MAPKKKK3, MEKKK 3, RAB8IPL1, KHS2
• Plasmid: pEBG6P-MAP4K3
• Parent Plasmid: pEBG6P
• Expressed: GST-MAP4K3
• Genbank: NP_003609.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAAT
ATACTACACTGCAAGATCTAATCTGGATCTGCAACTGGAATATGGACAAG
GACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCT
GTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGA
TGAAGGAGATGATGATGAATCTAAACACTCAACTCTGAAAGCAAAAATTC
CACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATG
CATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTC
AGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCA
GATTACCCCCACACAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTC
CAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATGAACA
TAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTA
TTAGTAATGGTCTTCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTT
TCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATG
GATAAACCCAGATACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAG
GGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAG
CTATTCCCTCGAAGGTGTACATGGTTGTATGTAATGAACAATTGCTTGCT
ATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGC
TTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCA
CACAAACTCCCTGACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAA
AATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTT
ACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTA
TTAGAATGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGA
TTTTCCTATACCATGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTG
AACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTC
AACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATG
GTTTACAGAATCAGATACCCCACAGACAAATGTTACTCATGTAACCCAAC
TGGAGAGAGATACCATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTA
AATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCAC
CTTTGATTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAG
CTTTCTGGAAACATGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTA
ACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGA
CAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATA
GCAATTTGTACATCCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNPGFDLSRRNPQ
EDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEII
MMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQI
AYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITA
TIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPP
MFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPT
AEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPH
RIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSE
EIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLE
DDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCP
MSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQN
EHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCAS
SWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNC
LLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVS
AKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKH
IDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTS
SWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSE
LTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLG
SDRVVVLESRPTDNPTANSNLYILAGHENSY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12242
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter GST for mammalian expression.; ; Estimated MW of expressed protein : 128731 Daltons