cDNA Clone MAP4K3

• Synonyms: GLK, MAPKKKK3, MEKKK 3, RAB8IPL1, KHS2
• Plasmid: pGEX6P-1-MAP4K3
• Parent Plasmid: pGEX6P1
• Expressed: GST-MAP4K3
• Genbank: NP_003609.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAAT
ATACTACACTGCAAGATCTAATCTGGATCTGCAACTGGAATATGGACAAG
GACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCT
GTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGA
TGAAGGAGATGATGATGAATCTAAACACTCAACTCTGAAAGCAAAAATTC
CACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATG
CATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTC
AGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCA
GATTACCCCCACACAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTC
CAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATGAACA
TAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTA
TTAGTAATGGTCTTCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTT
TCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATG
GATAAACCCAGATACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAG
GGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAG
CTATTCCCTCGAAGGTGTACATGGTTGTATGTAATGAACAATTGCTTGCT
ATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGC
TTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCA
CACAAACTCCCTGACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAA
AATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTT
ACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTA
TTAGAATGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGA
TTTTCCTATACCATGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTG
AACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTC
AACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATG
GTTTACAGAATCAGATACCCCACAGACAAATGTTACTCATGTAACCCAAC
TGGAGAGAGATACCATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTA
AATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCAC
CTTTGATTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAG
CTTTCTGGAAACATGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTA
ACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGA
CAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATA
GCAATTTGTACATCCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNPGFDLSRRNPQEDFELI
QRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCK
HPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRE
TLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRK
SFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHP
MRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQ
HPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTS
RNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTA
RSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDD
DESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPA
KPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTN
LSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPD
TRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISG
KASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPET
KWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIP
CPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTES
DTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQ
IESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVV
LESRPTDNPTANSNLYILAGHENSY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12241
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter GST for bacterial expression.; ; Estimated MW of expressed protein : 128137 Daltons