cDNA Clone MAP4K3

• Synonyms: GLK, MAPKKKK3, MEKKK 3, RAB8IPL1, KHS2
• Plasmid: pFBHTb MAP4K3
• Parent Plasmid: pFastBac HTb
• Expressed: 6His MAP4K3
• Genbank: NP_003609.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAAT
ATACTACACTGCAAGATCTAATCTGGATCTGCAACTGGAATATGGACAAG
GACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCT
GTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGA
TGAAGGAGATGATGATGAATCTAAACACTCAACTCTGAAAGCAAAAATTC
CACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATG
CATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTC
AGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCA
GATTACCCCCACACAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTC
CAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATGAACA
TAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTA
TTAGTAATGGTCTTCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTT
TCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATG
GATAAACCCAGATACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAG
GGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAG
CTATTCCCTCGAAGGTGTACATGGTTGTATGTAATGAACAATTGCTTGCT
ATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGC
TTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCA
CACAAACTCCCTGACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAA
AATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTT
ACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTA
TTAGAATGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGA
TTTTCCTATACCATGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTG
AACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTC
AACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATG
GTTTACAGAATCAGATACCCCACAGACAAATGTTACTCATGTAACCCAAC
TGGAGAGAGATACCATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTA
AATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCAC
CTTTGATTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAG
CTTTCTGGAAACATGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTA
ACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGA
CAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATA
GCAATTTGTACATCCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNPGFDLSRRNPQEDFELIQRI
GSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPN
IVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQ
GLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFI
GTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRA
LFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPF
VTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNV
REEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSN
LDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDES
KHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPS
QVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSR
KEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRD
QYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKAS
QLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWC
QKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPL
RMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTP
QTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIES
IVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLES
RPTDNPTANSNLYILAGHENSY*

• Vector Type: Baculovirus
• DU Number: DU12240
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 104685 Daltons