cDNA Clone BARD1

• Plasmid: pEBG6P-BARD1
• Parent Plasmid: pEBG6P
• Expressed: GST-BARD1
• Genbank: AAB38316.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGAT
CCGCTCCGGGAACGAGCCTCGTTCCGCGCCCGCCATGGAACCGGATGGTC
GCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTG
CTGCGCTGCTCGCGTTGTACTAACATTCTGAGAGAGCCTGTGTGTTTAGG
AGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAA
CTGGATGTCCAGTGTGTTACACCCCGGCCTGGATACAAGACTTGAAGATA
AATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTT
GCTACATGACAATGAGCTGTCAGATTTGAAAGAAGATAAACCTAGGAAAA
GTTTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTT
AGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCA
AACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATG
AATTTGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAG
GCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAAT
CAACCAAAAATGGAATTTAGAGGCAGAAAAAGAAGATGGTGAATTTGACT
CCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTTCTGTAGCCAACCATCT
GTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGG
CTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTAC
CACTGGCTGAGCAAATAGAGTCTCCAGACACTAAGAGCAGGAATGAAGTA
GTGACTCCTGAGAAGGTCTGCAAAAATTATCTTACATCTAAGAAATCTTT
GCCATTAGAAAATAATGGAAAACGTGGCCATCACAATAGACTTTCCAGTC
CCATTTCTAAGAGATGTAGAACCAGCATTCTGAGCACCAGTGGAGATTTT
GTTAAGCAAACGGTGCCCTCAGAAAATATACCATTGCCTGAATGTTCTTC
ACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGGAAAAACA
GTAACATGTCCGATGAATTCATTAGTCTTTCACCAGGTACACCACCTTCT
ACATTAAGTAGTTCAAGTTACAGGCAAGTGATGTCTAGTCCCTCAGCAAT
GAAGCTGTTGCCCAATATGGCTGTGAAAAGAAATCATAGAGGAGAGACTT
TGCTCCATATTGCTTCTATTAAGGGCGACATACCTTCTGTTGAATACCTT
TTACAAAATGGAAGTGATCCAAATGTTAAAGACCATGCTGGATGGACACC
ATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGC
TCCAGCATAAGGCATTGGTGAACACCACCGGGTATCAAAATGACTCACCA
CTTCACGATGCAGCCAAGAATGGGCACGTGGATATAGTCAAGCTGTTACT
TTCCTATGGAGCCTCCAGAAATGCTGTTAATATATTTGGTCTGCGGCCTG
TCGATTATACAGATGATGAAAGTATGAAATCGCTATTGCTGCTACCAGAG
AAGAATGAATCATCCTCAGCTAGCCACTGCTCAGTAATGAACACTGGGCA
GCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAAC
AACAGAAAATGCTCAGTGAGCTTGCAGTAATTCTTAAGGCTAAAAAATAT
ACTGAGTTTGACAGTACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGT
TCAAAGTACCTTGAAGTGTATGCTTGGGATTCTCAATGGATGCTGGATTC
TAAAATTTGAATGGGTAAAAGCATGTCTACGAAGAAAAGTATGTGAACAG
GAAGAAAAGTATGAAATTCCTGAAGGTCCACGCAGAAGCAGGCTCAACAG
AGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGG
GAACCTTCAAACACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCA
GGTGGGGGCCAGATCCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGAC
TCAGACCATCAATACAGTCGCATACCATGCGAGACCCGATTCTGATCAGC
GCTTCTGCACACAGTATATCATCTATGAAGATTTGTGTAATTATCACCCA
GAGAGGGTTCGGCAGGGCAAAGTCTGGAAGGCTCCTTCGAGCTGGTTTAT
AGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAGCGGCCG
C

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMPDN
RQPRNRQPRIRSGNEPRSAPAMEPDGRGAWAHSRAALDRLEKLLRCSRCT
NILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSM
IQLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKV
RYVVSKASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGK
KQKKKTLAEINQKWNLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQI
NGEIDLLASGSLTESECFGSLTEVSLPLAEQIESPDTKSRNEVVTPEKVC
KNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSILSTSGDFVKQTVPS
ENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLSSSSY
RQVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDP
NVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKN
GHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSA
SHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTV
THVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIP
EGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILS
RKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGK
VWKAPSSWFIDCVMSFELLPLDS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12160
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 114822 Daltons