cDNA Clone PPP2R1A

• Plasmid: pEBG6P-PPP2R1A
• Parent Plasmid: pEBG6P
• Expressed: GST-PPP2R1A
• Genbank: NP_055040.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGT
GCTCATAGACGAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACAGCA
TCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGACCCGA
AGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGT
CCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACCCTGGTGGGAG
GCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACA
GTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCAT
CTCACACGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAG
TGAAGCGGCTGGCGGGCGGCGACTGGTTCACCTCCCGCACCTCGGCCTGC
GGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGGCGGA
ACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGC
GGCGGGCCGCAGCCTCCAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTG
GACAACGTCAAGAGTGAGATCATCCCCATGTTCTCCAACCTGGCCTCTGA
CGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCG
CCCAGCTTCTGCCCCAGGAGGATCTGGAGGCCCTGGTGATGCCCACTCTG
CGCCAGGCCGCTGAAGACAAGTCCTGGCGCGTCCGCTACATGGTGGCTGA
CAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAGACAG
ACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTG
AGGGCCGCAGCCTCCCACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGC
TGACTGTCGGGAGAATGTGATCATGTCCCAGATCTTGCCCTGCATCAAGG
AGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTGCCCTGGCCTCAGTC
ATCATGGGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGCACCT
CTTGCCCCTCTTCCTGGCTCAGCTGAAGGATGAGTGCCCTGAGGTACGGC
TGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCATCCGG
CAGCTGTCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGC
CAAGTGGCGGGTGCGGCTGGCCATCATTGAGTACATGCCCCTCCTGGCTG
GACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTAACTCCTTGTGCATG
GCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCAGCAA
CCTGAAGAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAA
TCATCCCCAAGGTCTTGGCCATGTCCGGAGACCCCAACTACCTGCACCGC
ATGACTACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGCAGGA
CATCACCACCAAGCACATGCTACCCACGGTTCTGCGCATGGCTGGGGACC
CGGTTGCCAATGTCCGCTTCAATGTGGCCAAGTCTCTGCAGAAGATAGGG
CCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTCAAGCCCATCCTAGA
GAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGG
CTCTGACTGTTCTGTCTCTCGCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAAADGDDSLYPI
AVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDED
EVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLR
AISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVK
AELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLA
SDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMV
ADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENL
SADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIE
HLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAE
DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAAT
SNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCG
QDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPI
LEKLTQDQDVDVKYFAQEALTVLSLA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12155
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 92722 Daltons