cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-FLAG Ubiquitin K48wt
• Parent Plasmid: pGEX6P1
• Expressed: GST-FLAG Ubiquitin K48wt
• Genbank: P62988
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAGCGG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGKQLE
DGRTLSDYNIQRESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/FLAG for bacterial expression.