cDNA Clone Pellino2

• Plasmid: pCMV-HA-1 Pellino2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-Pellino 2
• Genbank: AAH09476.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGA
GCCAGTGAAATACGGGGAGCTGGTGGTGCTCGGGTACAATGGTGCTTTAC
CCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAAGCGG
CCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCC
CCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCCTACA
CTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGAT
ACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGT
TGTCACAGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCA
CACAGAGCACCATATCCAGGTTCGCCTGCAGGATCGTGTGCGACAGGAAT
GAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCAAAAA
CATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACA
TGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGC
TTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGG
AGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGC
TGGTGGAAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTG
TGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGGCTTTTTCATACTCC
AACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCCCGGC
CTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGG
AAAGAGGTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCA
CGTGCACGGGTACCACAACTGGGGCCATCGGAGTGACACGGAGGCCAACG
AGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGG
CTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGC
TTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATACTGGT
CTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCT
TTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTT
CCAAGGTCCAATTGACTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMFSPGQEEHCAPNKEPVKYGELVVLGYNGALPNGDRGR
RKSRFALYKRPKANGVKPSTVHVISTPQASKAISCKGQHSISYTLSRNQT
VVVEYTHDKDTDMFQVGRSTESPIDFVVTDTISGSQNTDEAQITQSTISR
FACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMDGLTTN
GVLVMHPRGGFTEESQPGVWREISVCGDVYTLRETRSAQQRGKLVESETN
VLQDGSLIDLCGATLLWRTADGLFHTPTQKHIEALRQEINAARPQCPVGL
NTLAFPSINRKEVVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMC
RTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCGHVCSEKSAKYWSQIPLPH
GTHAFHAACPFCATQLVGEQNCIKLIFQGPI

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV-HA-1 Pellino269.5 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12090
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 47677 Daltons
• Low Temp: No