cDNA Clone Ubiquitin

• Plasmid: pEBG6P-Ubiquitin K33wt
• Parent Plasmid: pEBG6P
• Expressed: GST-Ubiquitin K33wt
• Genbank: P62988
• Tag: GST
• Species: Human

Sequence of Insert

GGATCTCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAGCATGCAT
CTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCTCGCTGATC
AGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC
CCGTGCCTTCCTTGACCCTGGAAGGTGCCACAAGGGCGAATTCTGCAGAT
ATCCATCACACTGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSQIFVRTLTGRTIT
LEVEPSDTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRE
STLHLVLRLRGG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12070
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; ; Estimated MW of expressed protein : 35990 Daltons