cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pEBG6P-GSK3B wt
• Parent Plasmid: pEBG6P
• Expressed: GST-GSK3B wt
• Genbank: ABK42225.1
• Tag: GST

Sequence of Insert

GCGGCCGCGATGTCAGGGCGGCCCAGAACCACCTCCTTTGCGGAGAGCTG
CAAGCCGGTGCAGCAGCCTTCAGCTTTTGGCAGCATGAAAGTTAGCAGAG
ACAAGGACGGCAGCAAGGTGACAACAGTGGTGGCAACTCCTGGGCAGGGT
CCAGACAGGCCACAAGAAGTCAGCTATACAGACACTAAAGTGATTGGAAA
TGGATCATTTGGTGTGGTATATCAAGCCAAACTTTGTGATTCAGGAGAAC
TGGTCGCCATCAAGAAAGTATTGCAGGACAAGAGATTTAAGAATCGAGAG
CTCCAGATCATGAGAAAGCTAGATCACTGTAACATAGTCCGATTGCGTTA
TTTCTTCTACTCCAGTGGTGAGAAGAAAGATGAGGTCTATCTTAATCTGG
TGCTGGACTATGTTCCGGAAACAGTATACAGAGTTGCCAGACACTATAGT
CGAGCCAAACAGACGCTCCCTGTGATTTATGTCAAGTTGTATATGTATCA
GCTGTTCCGAAGTTTAGCCTATATCCATTCCTTTGGAATCTGCCATCGGG
ATATTAAACCGCAGAACCTCTTGTTGGATCCTGATACTGCTGTATTAAAA
CTCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCGAGGAGAACCCAATGT
TTCGTATATCTGTTCTCGGTACTATAGGGCACCAGAGTTGATCTTTGGAG
CCACTGATTATACCTCTAGTATAGATGTATGGTCTGCTGGCTGTGTGTTG
GCTGAGCTGTTACTAGGACAACCAATATTTCCAGGGGATAGTGGTGTGGA
TCAGTTGGTAGAAATAATCAAGGTCCTGGGAACTCCAACAAGGGAGCAAA
TCAGAGAAATGAACCCAAACTACACAGAATTTAAATTCCCTCAAATTAAG
GCACATCCTTGGACTAAGGTCTTCCGACCCCGAACTCCACCGGAGGCAAT
TGCACTGTGTAGCCGTCTGCTGGAGTATACACCAACTGCCCGACTAACAC
CACTGGAAGCTTGTGCACATTCATTTTTTGATGAATTACGGGACCCAAAT
GTCAAACTACCAAATGGGCGAGACACACCTGCACTCTTCAACTTCACCAC
TCAAGAACTGTCAAGTAATCCACCTCTGGCTACCATCCTTATTCCTCCTC
ATGCTCGGATTCAAGCAGCTGCTTCAACCCCCACAAATGCCACAGCAGCG
TCAGATGCTAATACTGGAGACCGTGGACAGACCAATAATGCTGCTTCTGC
ATCAGCTTCCAACTCCACCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMSGR
PRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEV
SYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKL
DHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLP
VIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSA
KQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQ
PIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKV
FRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGR
DTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGD
RGQTNNAASASASNST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12065
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 74947 Daltons