cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pEBG6P-GSK3B INS
• Parent Plasmid: pEBG6P
• Expressed: GST-GSK3B INS
• Genbank: ABK42225.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCAGGGCGGCCCAGAACCACCTCCTTTGCGGAGAGCTG
CAAGCCGGTACAGCAGCCTTCAGCTTTTGGCAGCATGAAAGTTAGCAGAG
ACAAGGACGGCAGCAAGGTGACAACAGTGGTGGCAACTCCTGGGCAGGGT
CCAGACAGGCCACAAGAAGTCAGCTATACAGACACTAAAGTGATTGGAAA
TGGATCATTTGGTGTGGTATATCAAGCCAAACTTTGTGATTCAGGAGAAC
TGGTCGCCATCAAGAAAGTATTGCAGGACAAGAGATTTAAGAATCGAGAG
CTCCAGATCATGAGAAAGCTAGATCACTGTAACATAGTCCGATTGCGTTA
TTTCTTCTACTCCAGTGGTGAGAAGAAAGATGAGGTCTATCTTAATCTGG
TGCTGGACTATGTTCCGGAAACAGTATACAGAGTTGCCAGACACTATAGT
CGAGCCAAACAGACGCTCCCTGTGATTTATGTCAAGTTGTATATGTATCA
GCTGTTCCGAAGTTTAGCCTATATCCATTCCTTTGGAATCTGCCATCGGG
ATATTAAACCGCAGAACCTCTTGTTGGATCCTGATACTGCTGTATTAAAA
CTCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCGAGGAGAACCCAATGT
TTCGTATATCTGTTCTCGGTACTATAGGGCACCAGAGTTGATCTTTGGAG
CCACTGATTATACCTCTAGTATAGATGTATGGTCTGCTGGCTGTGTGTTG
GCTGAGCTGTTACTAGGACAACCAATATTTCCAGGGGATAGTGGTGTGGA
TCAGTTGGTAGAAATAATCAAGGTCCTGGGAACTCCAACAAGGGAGCAAA
TCAGAGAAATGAACCCAAACTACACAGAATTTAAATTCCCTCAAATTAAG
GCACATCCTTGGACTAAGGATTCGTCAGGAACAGGACATTTCACCTCAGG
AGTGCGGGTCTTCCGACCCCGAACTCCACCGGAGGCAATTGCACTGTGTA
GCCGTCTGCTGGAGTATACACCAACTGCCCGACTAACACCACTGGAAGCT
TGTGCACATTCATTTTTTGATGAATTACGGGACCCAAATGTCAAACTACC
AAATGGGCGAGACACACCTGCACTCTTCAACTTCACCACTCAAGAACTGT
CAAGTAATCCACCTCTGGCTACCATCCTTATTCCTCCTCATGCTCGGATT
CAAGCAGCTGCTTCAACCCCCACAAATGCCACAGCAGCGTCAGATGCTAA
TACTGGAGACCGTGGACAGACCAATAATGCTGCTTCTGCATCAGCTTCCA
ACTCCACCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMSGR
PRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEV
SYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKL
DHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLP
VIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSA
KQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQ
PIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKD
SSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFD
ELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTP
TNATAASDANTGDRGQTNNAASASASNST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12064
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 76236 Daltons