cDNA Clone DDX20

• Plasmid: pACT2.6 DDX20 1-716
• Parent Plasmid: pACT2.6
• Expressed: GAL4 DNA AD-DDX20 1-716
• Genbank: AAH34953.1
• Tag: GAL4 DNA-AD
• Species: Human

Sequence of Insert

AGATCTATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCAGCAGTGGC
GACTGCTATGCCGGCTGAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGC
CAACACCCGGGCCTGTGAGGATCCTGCGGACCGCTCAGGATCTCAGCAGC
CCGCGGACCCGCACGGGGGATGTGCTGTTGGCGGAGCCGGCCGACTTCGA
GTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGCTGCGGGCGGCCGGCT
TCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGCTGGGGCGCTGC
GGGCTCGATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGT
GTTCTCCACCATAGCTTTGGACTCTCTTGTTCTTGAAAACTTAAGTACCC
AGATTTTGATCTTGGCTCCTACAAGAGAAATTGCTGTACAGATACATTCT
GTTATTACAGCCATTGGAATAAAAATGGAAGGCTTAGAGTGTCATGTCTT
TATTGGAGGGACCCCATTATCACAAGACAAAACCAGACTTAAAAAGTGTC
ATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAACTTGAC
TACTTGAACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAA
GCTTTTAGAAGAAGGCAGCTTCCAGGAGCAAATAAATTGGATTTATTCTT
CCTTGCCTGCCAGTAAACAGATGCTGGCAGTATCAGCTACTTATCCCGAA
TTTTTGGCTAATGCTTTGACAAAGTACATGAGAGATCCCACTTTTGTAAG
ACTGAATTCCAGTGATCCAAGTCTCATAGGTTTGAAGCAGTATTACAAAG
TTGTCAATTCATACCCTTTGGCACATAAGGTTTTTGAGGAAAAGACTCAG
CATTTACAGGAACTGTTCAGCAGAATTCCATTTAATCAAGCTTTAGTCTT
TTCTAATTTGCACAGCAGAGCACAACATTTGGCTGATATCCTTTCTTCTA
AAGGCTTTCCTGCTGAGTGCATTTCAGGCAATATGAATCAGAATCAGCGT
CTTGATGCTATGGCTAAACTGAAGCACTTTCATTGCAGAGTCCTCATTTC
CACAGATTTGACTTCTCGTGGGATTGATGCTGAGAAGGTGAATCTGGTTG
TAAATCTGGATGTACCATTGGATTGGGAGACATACATGCATCGGATTGGG
AGAGCTGGCCGTTTTGGTACATTGGGGCTGACAGTGACCTACTGTTGCCG
GGGAGAGGAAGGAAATATGATGATGAGAATTGCCCAGAAATGTAATATCA
ACCTTCTCCCTTTACCAGATCCCATTCCTTCTGGTCTGATGGAAGAATGT
GTGGATTGGGATGTGGAAGTTAAAGCTGCTGTGCATACATATGGTATAGC
AAGTGTACCTAACCAACCCTTAAAAAAGCAAATTCAGAAAATAGAGAGAA
CCCTTCAAATTCAGAAAGCTCATGGTGACCACATGGCTTCCTCTAGAAAT
AATTCTGTATCTGGACTATCAGTCAAATCAAAAAATAATACCAAACAAAA
GCTTCCTGTGAAAAGCCACTCAGAATGTGGAATCATAGAAAAAGCAACGT
CACCAAAAGAACTGGGCTGTGACAGGCAATCCGAAGAGCAAATGAAGAAT
TCTGTTCAGACTCCCGTTGAAAACTCCACCAACAGTCAGCACCAGGTCAA
AGAAGCTTTACCTGTGTCACTCCCCCAGATTCCTTGTCTGTCTTCCTTTA
AAATCCATCAGCCATACACGTTGACTTTTGCTGAATTGGTAGAGGATTAT
GAACATTATATTAAAGAGGGGTTAGAGAAACCTGTGGAAATCATCAGGCA
CTACACAGGCCCTGGGGATCAGACTGTGAATCCTCAAAATGGTTTTGTGA
GAAATAAAGTTACTGAACAGAGAGTCCCTGTATTGGCAAGTAGTAGCCAA
TCTGGAGACTCTGAGAGTGACAGTGATTCTTACAGCTCAAGAACCTCTTC
CCAGAGCAAAGGAAATAAGTCATACTTGGAAGGCTCTTCTGATAATCAGC
TGAAAGACTCTGAATCTACGCCTGTGGATGATCGTATTTCTTTGGAACAA
CCACCAAATGGAAGTGACACCCCCAATCCAGAGAAATATCAAGAATCACC
TGGGTAACAGATGAAGACAAGACTTAAAGAGGGGGCTAGCCAGAGAGCTA
AGCAGAGCCGGAGAAACCTACCCAGGCGGTCTTCCTTCAGATTGCAGACT
GAAGCCCAGGAAGATGATTGGTATGACTGTCATAGGGAAATACGTCTGAG
TTTTTCTGATACCTATCAGGATTATGAGGAGTACTGGAGAGCTTACTACA
GGGCATGGCAAGAATATTATGCTGCCGCTTCTCATTCATATTATTGGAAT
GCTCAGAGACATCCAAGTTGGATGGCAGCTTATCACATGAATACCATTTA
TCTACAAGAAATGATGCATAGTAACCAGTGACTCGAG

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYAS
LGGHMAMGGSMAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRIL
RTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQL
KAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTR
EIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGR
IKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQML
AVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAH
KVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECIS
GNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDW
ETYMHRIGRAGRFGTLGLTVTYCCRGEEGNMMMRIAQKCNINLLPLPDPI
PSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHG
DHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDR
QSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLT
FAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQRV
PVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPV
DDRISLEQPPNGSDTPNPEKYQESPG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU12055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Y2H construct.; ; Estimated MW of expressed protein : 92153 Daltons