cDNA Clone CBL

• Plasmid: pGEX6P-1-CBL
• Parent Plasmid: pGEX6P1
• Expressed: GST-CBL
• Genbank: P22681
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCGGCAACGTGAAGAAGAGCTCTGGGGCCGGGGGCGGCAG
CGGCTCCGGGGGCTCGGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACG
CCTTCCAGCCGCACCACCACCACCACCACCACCTCAGCCCCCACCCGCCG
GGGACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAA
GGTGGTGCGGTTGTGTCAGAACCCAAAGCTGGCGCTAAAGAATAGCCCAC
CTTATATCTTAGACCTGCTACCAGATACCTACCAGCATCTCCGTACTATC
TTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATTT
TAGGGTGTTTATGGAGAATTTGATGAAGAAAACTAAGCAAACCATAAGCC
TCTTCAAGGAGGGAAAAGAAAGAATGTATGAGGAGAATTCTCAGCCTAGG
CGAAACCTAACCAAACTGTCCCTCATCTTCAGCCACATGCTGGCAGAACT
AAAAGGAATCTTTCCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTA
CTAAAGCAGATGCTGCGGAATTTTGGAGAAAAGCTTTTGGGGAAAAGACA
ATAGTCCCTTGGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCAT
CAGTTCTGGGCTGGAGGCCATGGCTCTGAAATCCACTATTGATCTGACCT
GCAATGATTATATTTCGGTTTTTGAATTTGACATCTTTACCCGACTCTTT
CAGCCCTGGTCCTCTTTGCTCAGGAATTGGAACAGCCTTGCTGTAACTCA
TCCTGGCTACATGGCTTTTTTGACGTATGACGAAGTGAAAGCTCGGCTCC
AGAAATTCATTCACAAACCTGGCAGTTATATCTTCCGGCTGAGCTGTACT
CGTCTGGGTCAGTGGGCTATTGGGTATGTTACTGCTGATGGGAACATTCT
CCAGACAATCCCTCACAATAAACCTCTCTTCCAAGCACTGATTGATGGCT
TCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAGAATCCTGAT
CTGACTGGCTTATGTGAACCAACTCCCCAAGACCATATCAAAGTGACCCA
GGAACAATATGAATTATACTGTGAGATGGGCTCCACATTCCAACTATGTA
AAATATGTGCTGAAAATGATAAGGATGTAAAGATTGAGCCCTGTGGACAC
CTCATGTGCACATCCTGTCTTACATCCTGGCAGGAATCAGAAGGTCAGGG
CTGTCCTTTCTGCCGATGTGAAATTAAAGGTACTGAACCCATCGTGGTAG
ATCCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAG
GGAGCTCCCTCCCCAAATTATGATGATGATGATGATGAACGAGCTGATGA
TACTCTCTTCATGATGAAGGAATTGGCTGGTGCCAAGGTGGAACGGCCGC
CTTCTCCATTCTCCATGGCCCCACAAGCTTCCCTTCCCCCGGTGCCACCA
CGACTTGACCTTCTGCCGCAGCGAGTATGTGTTCCCTCAAGTGCTTCTGC
TCTTGGAACTGCTTCTAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAAC
CATTGCCAGTACCTCCCACACTTCGAGATCTTCCACCACCACCGCCTCCA
GACCGGCCATATTCTGTTGGAGCAGAATCCCGACCTCAAAGACGCCCCTT
GCCTTGTACACCAGGCGACTGTCCCTCCAGAGACAAACTGCCCCCTGTCC
CCTCTAGCCGCCTTGGAGACTCATGGCTGCCCCGGCCAATCCCCAAAGTA
CCAGTATCTGCCCCAAGTTCCAGTGATCCCTGGACAGGAAGAGAATTAAC
CAACCGGCACTCACTTCCATTTTCATTGCCCTCACAAATGGAGCCCAGAC
CAGATGTGCCTAGGCTCGGAAGCACGTTCAGTCTGGATACCTCCATGAGT
ATGAATAGCAGCCCATTAGTAGGTCCAGAGTGTGACCACCCCAAAATCAA
ACCTTCCTCATCTGCCAATGCCATTTATTCTCTGGCTGCCAGACCTCTTC
CTGTGCCAAAACTGCCACCTGGGGAGCAATGTGAGGGTGAAGAGGACACA
GAGTACATGACTCCCTCTTCCAGGCCTCTACGGCCTTTGGATACATCCCA
GAGTTCACGAGCATGTGATTGCGACCAGCAGATTGATAGCTGTACGTATG
AAGCAATGTATAATATTCAGTCCCAGGCGCCATCTATCACCGAGAGCAGC
ACCTTTGGTGAAGGGAATTTGGCCGCAGCCCATGCCAACACTGGTCCCGA
GGAGTCAGAAAATGAGGATGATGGGTATGATGTCCCAAAGCCACCTGTGC
CGGCCGTGCTGGCCCGCCGAACTCTCTCAGATATCTCTAATGCCAGCTCC
TCCTTTGGCTGGTTGTCTCTGGATGGTGATCCTACAACAAATGTCACTGA
AGGTTCCCAAGTTCCCGAGAGGCCTCCAAAACCATTCCCGCGGAGAATCA
ACTCTGAACGGAAAGCTGGCAGCTGTCAGCAAGGTAGTGGTCCTGCCGCC
TCTGCTGCCACCGCCTCACCTCAGCTCTCCAGTGAGATCGAGAACCTCAT
GAGTCAGGGGTACTCCTACCAGGACATCCAGAAAGCTTTGGTCATTGCCC
AGAACAACATCGAGATGGCCAAAAACATCCTCCGGGAATTTGTTTCCATT
TCTTCTCCTGCCCATGTAGCTACCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGNVKKSSGAGGGSGSGG
SGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRL
CQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFM
ENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIF
PSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGL
EAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYM
AFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIP
HNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYE
LYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFC
RCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFM
MKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTA
SKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTP
GDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHS
LPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSS
ANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRA
CDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESEN
EDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQV
PERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGY
SYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12029
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 126453 Daltons