cDNA Clone Ubiquitin

• Plasmid: pIRES3 FLAG Ubiquitin K29wt
• Parent Plasmid: pIRESpuro3
• Expressed: FLAG Ubiquitin K29wt
• Genbank: P62988
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGGATCTGCCACCATGGACTACAAGGACGACGATGACAAGCAAAT
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAGGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACCACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCTTAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MDYKDDDDKQIFVRTLTGRTITLEVEPSDTIENVRAKIQDREGIPPDQQR
LIFAGRQLEDGRTLSDYHIQRESTLHLVLRLLGG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8963
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG for mammalian stable expression.