cDNA Clone MEKK2

• Synonyms: MAP3K2
• Plasmid: pCMV-HA MEKK2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-MEKK2
• Genbank: XP_001128799.1
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGATGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGTTAAACTGGAAGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAATCTATGGATCTACATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTGGACAAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGATATTACTTGTAATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGATAATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGAGATAGAAGTTCTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAATGGGTCATTCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATGCTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGTTGTCCATCACTTGATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAATGCCTAGGGCTCAGAGCTACCCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAAATTTGGAAAAGGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGTCGTAAAACTTTTCCAAGAGCCAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGTTTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAGTATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTAATGGACATCAGCCCACCCAGCCGTTCACCTCGAGCTCCGACCAACTGGAGATTGGGCAAACTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGAGAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAAGTAAATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAGTATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATGCCAGGGGGTTCAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACTAGGAAATACACCCGTCAGATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTCCATAGAGATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTAGGAGATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGACAGGAATGAAGTCTGTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGGCTATGGAAGAAAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCTTGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCAAAGCTGCCACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGTGCATTATCACTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSPEFDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSS
SPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTN
NELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSL
EDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSI
NSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPR
AQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPR
ARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNP
TLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQ
VQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIF
MEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKG
ANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGE
GYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHV
SDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8953
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 79080 Daltons
• Low Temp: No