cDNA Clone PPP4R2

• Plasmid: pGEX6P-2-PPP4R2 FL
• Parent Plasmid: pGEX6P2
• Expressed: GST-PPP4R2 FL
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTGCCAGGCGCCATGTTGGAGGGCTGGTGGTAGCGGCTT
GGGGAGGTGCTCACTCTGTCGGTCTTGCTCTCTCGCACGCTTCCCCCGGC
TCCCTTCGTTTCCCCCCCCCGGTCGCCTGCGTGCCGGAGTGTGTGCGAGG
GAGGGGGAGGGCGTCGGGGGGGTGGGGGGAGGCGTTCCGGTCCCCAAAAG
ACCCGCGGAGGGAGGCGGAGGCTGTGAGGGACTCCGGGAAGCCATGGACG
TCGAGAGGCTCCAGGAGGCGCTGAAAGATTTTGAGAAGAGGGGGAAAAAG
GAAGTTTGTCCTGTCCTGGATCAGTTTCTTTGTCATGTAGCCAAGACTGG
AGAAACAATGATTCAGTGGTCCCAATTTAAAGGCTATTTTATTTTCAAAC
TGGAGAAAGTGATGGATGATTTCAGAACTTCAGCTCCTGAGCCAAGAGGT
CCTCCCAACCCTAATGTCGAATATATTCCCTTTGATGAAATGAAGGAAAG
AATACTGAAAATTGTCACTGGATTTAATGGTATCCCTTTTACTATTCAGC
GACTATGTGAATTGTTAACAGATCCAAGGAGAAACTATACAGGAACAGAC
AAATTTCTCAGAGGAGTAGAAAAGAATGTGATGGTTGTTAGCTGTGTTTA
TCCTTCTTCAGAGAAAAACAATTCCAATAGTTTAAATCGAATGAATGGTG
TTATGTTTCCTGGAAATTCACCAAGCTATACTGAGAGGTCTAATATAAAT
GGGCCTGGGACACCCAGGCCACTTAATCGACCAAAGGTTTCTTTGTCAGC
CCCCATGACAACAAATGGGTTGCCTGAGAGCACAGACAGCAAAGAGGCAA
ATTTGCAGCAAAATGAGGAGAAAAATCACAGTGACTCTTCGACCTCTGAA
TCAGAAGTTTCCTCAGTGAGCCCTTTGAAAAATAAACATCCAGATGAAGA
TGCTGTGGAAGCTGAGGGGCATGAGGTAAAAAGACTCAGGTTTGACAAAG
AAGGCGAAGTCAGAGAAACAGCCAGTCAAACGACTTCCAGCGAAATTTCT
TCAGTTATGGTAGGAGAAACAGAAGCATCATCTTCATCTCAGGATAAAGA
CAAAGATAGCCGTTGTACCCGGCAGCACTGTACAGAAGAGGATGAAGAAG
AGGATGAAGAGGAAGAAGAAGAGTCTTTTATGACATCAAGAGAAATGATC
CCAGAAAGAAAAAATCAAGAAAAAGAATCTGATGATGCCTTAACTGTGAA
TGAAGAGACTTCTGAGGAAAATAATCAAATGGAGGAATCTGATGTGTCTC
AAGCTGAGAAAGATTTGCTACATTCTGAAGGTAGTGAAAACGAAGGCCCT
GTAAGTAGTAGTTCTTCTGACTGCCGTGAAACAGAAGAATTAGTAGGATC
CAATTCCAGTAAAACTGGAGAGATTCTTTCAGAATCATCCATGGAAAATG
ATGACGAAGCCACAGAAGTCACCGATGAACCAATGGAACAAGACTAAGCG
GCCGCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMCQAPCWR
AGGSGLGRCSLCRSCSLARFPRLPSFPPPGRLRAGVCAREGEGVGGVGGG
VPVPKRPAEGGGGCEGLREAMDVERLQEALKDFEKRGKKEVCPVLDQFLC
HVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPF
DEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVM
VVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRP
KVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKN
KHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGETEASS
SSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQEKESD
DALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSDCRET
EELVGSNSSKTGEILSESSMENDDEATEVTDEPMEQD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8933
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 82505 Daltons