cDNA Clone TIE2

• Plasmid: pGEX6P-2-TIE2
• Parent Plasmid: pGEX6P2
• Expressed: GST-TIE2
• Genbank: AAH35514.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTT
GCTCCTTTCTGGAACTGTGGAAGGTGCCATGGACTTGATCTTGATCAATT
CCCTACCTCTTGTATCTGATGCTGAAACATCTCTCACCTGCATTGCCTCT
GGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTT
AATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGAG
AATGGGCTAAAAAAGTTGTCTGGAAGAGAGAAAAGGCTAGTAAGATCAAT
GGTGCTTATTTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACG
AACCATGAAGATGCGTCAACAAGCTTCCTTCCTACCAGCTACTTTAACTA
TGACTGTGGACAAGGGAGATAACGTGAACATATCTTTCAAAAAGGTATTG
ATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATCCATTC
AGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCATG
CTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAAC
CTCTTCACCTCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCA
GAAGTGGGGACCTGAATGCAACCATCTCTGTACTGCTTGTATGAACAATG
GTGTCTGCCATGAAGATACTGGAGAATGCATTTGCCCTCCTGGGTTTATG
GGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTTGGCAGAACTTG
TAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGTC
TCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAG
TGCAATGAAGCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAG
GTGCAGCTGCAACAATGGGGAGATGTGTGATCGTTTCCAAGGATGTCTCT
GCTCTCCAGGATGGCAGGGGCTCCAGTGTGAGAGAGAAGGCATACCGAGG
ATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAAGTAAACAGTGG
TAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAATG
AAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGAC
TTTAACCATACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGAT
CCTCCCCCCTGACTCAGGAGTTTGGGTCTGCAGTGTGAACACAGTGGCTG
GGATGGTGGAAAAGCCCTTCAACATTTCTGTTAAAGTTCTTCCAAAGCCC
CTGAATGCCCCAAACGTGATTGACACTGGACATAACTTTGCTGTCATCAA
CATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAGC
TTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTG
ACAAATGAGATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGA
ACTCTGTGTGCAACTGGTCCGTCGTGGAGAGGGTGGGGAAGGGCATCCTG
GACCTGTGAGACGCTTCACAACAGCTTCTATCGGACTCCCTCCTCCAAGA
GGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAATTTGACCTGGCA
ACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGAA
GGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTG
ACTTCGGTGCTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCG
AGCTAGAGTCAACACCAAGGCCCAGGGGGAATGGAGTGAAGATCTCACTG
CTTGGACCCTTAGTGACATTCTTCCTCCTCAACCAGAAAACATCAAGATT
TCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACAATATTGGATGG
CTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAATG
AAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCACTCAGTAT
CAGCTCAAGGGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGC
AGAGAACAACATAGGGTCAAGCAACCCAGCCTTTTCTCATGAACTGGTGA
CCCTCCCAGAATCTCAAGCACCAGCGGACCTCGGAGGGGGGAAGATGCTG
CTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTGACTGTGCTGTT
GGCCTTTCTGATCATATTGCAATTGAAGAGAGCAAATGTGCAAAGGAGAA
TGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAAC
TCAGGGACTCTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTAC
AATTTATCCAGTGCTTGACTGGAATGACATCAAATTTCAAGATGTGATTG
GGGAGGGCAATTTTGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGG
TTACGGATGGATGCTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGA
TGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGAC
ACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTAC
TTGTACCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCT
TCGCAAGAGCCGTGTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATA
GCACCGCGTCCACACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGAC
GTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGA
TCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAG
CAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATG
GGAAGGCTCCCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGT
GTACACAACCAACAGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGA
TTGTTAGCTTAGGAGGCACACCCTACTGCGGGATGACTTGTGCAGAACTC
TACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGA
TGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATG
AGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAG
GAGCGAAAGACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGC
AGGAATTGACTGTTCTGCTGAAGAAGCGGCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDSLASLV
LCGVSLLLSGTVEGAMDLILINSLPLVSDAETSLTCIASGWRPHEPITIG
RDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRG
EAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKN
GSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIV
RRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELH
TFGRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYG
PDCKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIPRMTPKIVDLPDH
IEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAI
FTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGH
NFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLE
PRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTT
LNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPR
EQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVIS
WTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAY
QVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLIAILGSAGMT
CLTVLLAFLIILQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKVK
NNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMK
EYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHG
NLLDFLRKSRVLETDPAFAIANSTASTLSSHHLLHFAADVARGMDYLSQK
QFIHRDLAARNILVGENYVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIE
SLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLE
KPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLY
EKFTYAGIDCSAEEAA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8932
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 153615 Daltons