cDNA Clone IRAK4

• Plasmid: pFastbac GST IRAK4 D329A
• Parent Plasmid: pFastbac GST
• Expressed: GST-IRAK4 D329A
• Genbank: AAH13316.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAA
TGTTGGACTAATTAGGAAGCTGTCAGATTTTATTGATCCTCAAGAAGGAT
GGAAGAAGTTAGCTGTAGCTATTAAAAAACCATCTGGTGATGATAGATAC
AATCAGTTTCACATAAGGAGATTTGAAGCATTACTTCAAACTGGAAAAAG
TCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACAGTTG
GTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCAAGT
CTTTTGCTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAA
AGAAGCTATAACAGTTCAGCAAAAACAGATGCCTTTCTGTGACAAAGACA
GGACATTGATGACACCTGTGCAGAATCTTGAACAAAGCTATATGCCACCT
GACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTTAGTGATACACGTTT
TCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTTGATG
AACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGA
GTTGTATATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCT
TGCAGCAATGGTTGACATTACTACTGAAGAACTGAAACAGCAGTTTGATC
AAGAAATAAAAGTAATGGCAAAGTGTCAACATGAAAACTTAGTAGAACTA
CTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTAGTATATGTTTACAT
GCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACTCCAC
CACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGC
ATCAATTTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGC
AAATATCTTACTGGATGAAGCTTTTACTGCTAAAATATCTGCCTTTGGCC
TTGCACGGGCTTCTGAGAAGTTTGCCCAGACAGTCATGACTAGCAGAATT
GTGGGAACAACAGCTTATATGGCACCAGAAGCTTTGCGTGGAGAAATAAC
ACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATAATAA
CTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGAT
ATTAAAGAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGA
TAAAAAGATGAATGATGCTGATTCCACTTCAGTTGAAGCTATGTACTCTG
TTGCTAGTCAATGTCTGCATGAAAAGAAAAATAAGAGACCAGACATTAAG
AAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNKPITPSTYVRCLNVGLI
RKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSE
LLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAIT
VQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFS
FYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMV
DITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGS
LLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILL
DEAFTAKISAFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSD
IYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMN
DADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8930
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression (kinase dead); ; Estimated MW of expressed protein : 78307 Daltons