cDNA Clone DDX20

• Plasmid: pGEX6P-1-DDX20
• Parent Plasmid: pGEX6P1
• Expressed: GST-DDX20
• Genbank: AAH34953.1
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCAGCAGTGGC
GACTGCTATGCCGGCTGAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGC
CAACACCCGGGCCTGTGAGGATCCTGCGGACCGCTCAGGATCTCAGCAGC
CCGCGGACCCGCACGGGGGATGTGCTGTTGGCGGAGCCGGCCGACTTCGA
GTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGCTGCGGGCGGCCGGCT
TCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGCTGGGGCGCTGC
GGGCTCGATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGT
GTTCTCCACCATAGCTTTGGACTCTCTTGTTCTTGAAAACTTAAGTACCC
AGATTTTGATCTTGGCTCCTACAAGAGAAATTGCTGTACAGATACATTCT
GTTATTACAGCCATTGGAATAAAAATGGAAGGCTTAGAGTGTCATGTCTT
TATTGGAGGGACCCCATTATCACAAGACAAAACCAGACTTAAAAAGTGTC
ATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAACTTGAC
TACTTGAACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAA
GCTTTTAGAAGAAGGCAGCTTCCAGGAGCAAATAAATTGGATTTATTCTT
CCTTGCCTGCCAGTAAACAGATGCTGGCAGTATCAGCTACTTATCCCGAA
TTTTTGGCTAATGCTTTGACAAAGTACATGAGAGATCCCACTTTTGTAAG
ACTGAATTCCAGTGATCCAAGTCTCATAGGTTTGAAGCAGTATTACAAAG
TTGTCAATTCATACCCTTTGGCACATAAGGTTTTTGAGGAAAAGACTCAG
CATTTACAGGAACTGTTCAGCAGAATTCCATTTAATCAAGCTTTAGTCTT
TTCTAATTTGCACAGCAGAGCACAACATTTGGCTGATATCCTTTCTTCTA
AAGGCTTTCCTGCTGAGTGCATTTCAGGCAATATGAATCAGAATCAGCGT
CTTGATGCTATGGCTAAACTGAAGCACTTTCATTGCAGAGTCCTCATTTC
CACAGATTTGACTTCTCGTGGGATTGATGCTGAGAAGGTGAATCTGGTTG
TAAATCTGGATGTACCATTGGATTGGGAGACATACATGCATCGGATTGGG
AGAGCTGGCCGTTTTGGTACATTGGGGCTGACAGTGACCTACTGTTGCCG
GGGAGAGGAAGGAAATATGATGATGAGAATTGCCCAGAAATGTAATATCA
ACCTTCTCCCTTTACCAGATCCCATTCCTTCTGGTCTGATGGAAGAATGT
GTGGATTGGGATGTGGAAGTTAAAGCTGCTGTGCATACATATGGTATAGC
AAGTGTACCTAACCAACCCTTAAAAAAGCAAATTCAGAAAATAGAGAGAA
CCCTTCAAATTCAGAAAGCTCATGGTGACCACATGGCTTCCTCTAGAAAT
AATTCTGTATCTGGACTATCAGTCAAATCAAAAAATAATACCAAACAAAA
GCTTCCTGTGAAAAGCCACTCAGAATGTGGAATCATAGAAAAAGCAACGT
CACCAAAAGAACTGGGCTGTGACAGGCAATCCGAAGAGCAAATGAAGAAT
TCTGTTCAGACTCCCGTTGAAAACTCCACCAACAGTCAGCACCAGGTCAA
AGAAGCTTTACCTGTGTCACTCCCCCAGATTCCTTGTCTGTCTTCCTTTA
AAATCCATCAGCCATACACGTTGACTTTTGCTGAATTGGTAGAGGATTAT
GAACATTATATTAAAGAGGGGTTAGAGAAACCTGTGGAAATCATCAGGCA
CTACACAGGCCCTGGGGATCAGACTGTGAATCCTCAAAATGGTTTTGTGA
GAAATAAAGTTACTGAACAGAGAGTCCCTGTATTGGCAAGTAGTAGCCAA
TCTGGAGACTCTGAGAGTGACAGTGATTCTTACAGCTCAAGAACCTCTTC
CCAGAGCAAAGGAAATAAGTCATACTTGGAAGGCTCTTCTGATAATCAGC
TGAAAGACTCTGAATCTACGCCTGTGGATGATCGTATTTCTTTGGAACAA
CCACCAAATGGAAGTGACACCCCCAATCCAGAGAAATATCAAGAATCACC
TGGGATCCAGATGAAGACAAGACTTAAAGAGGGGGCTAGCCAGAGAGCTA
AGCAGAGCCGGAGAAACCTACCCAGGCGGTCTTCCTTCAGATTGCAGACT
GAAGCCCAGGAAGATGATTGGTATGACTGTCATAGGGAAATACGTCTGAG
TTTTTCTGATACCTATCAGGATTATGAGGAGTACTGGAGAGCTTACTACA
GGGCATGGCAAGAATATTATGCTGCCGCTTCTCATTCATATTATTGGAAT
GCTCAGAGACATCCAAGTTGGATGGCAGCTTATCACATGAATACCATTTA
TCTACAAGAAATGATGCATAGTAACCAGTGACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAAFEASGALAAVATAMP
AEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLL
SRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTI
ALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGT
PLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEE
GSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSS
DPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLH
SRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLT
SRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEG
NMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPN
QPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVK
SHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALP
VSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGP
GDQTVNPQNGFVRNKVTEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKG
NKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQM
KTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDT
YQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEM
MHSNQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8920
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 118976 Daltons