cDNA Clone IRAK1

• Plasmid: pGEX6P-2-IRAK1 D358A new
• Parent Plasmid: pGEX6P2
• Expressed: GST-IRAK1 D358A new
• Genbank: NP_001560.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGCAGCCCCCGG
CGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCT
ACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCCGCC
CTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCA
GCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACGCCCGTGTGG
CCGACCTGGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGAC
ATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCCAGGCACCAC
TGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGA
GCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCCCCAGCTTTT
CCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCC
TGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTTCTACCAAGC
CAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTTTCCG
TTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGA
GGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGA
TGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAACGCTGACCTG
GAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTC
CAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGA
ACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAG
GACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCA
GCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATC
AGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTT
CTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTT
CAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGA
CACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAG
ACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGT
GCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGA
CCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTG
GCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGC
CTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGACCCCA
GGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCC
TGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGT
GTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGC
ATTGGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTAC
GTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCT
GGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAG
GCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCT
GCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACC
CCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCT
GGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTT
GGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCC
TGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCC
TGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTG
GAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAGGPGPG
EPAAPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRL
CERSGQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPL
PSPGTTAPRPSSIPAPAEAEAWSPRKLPSSASTFLSPAFPGSQTHSGPEL
GLVPSPASLWPPPPSPAPSSTKPGPESSVSLLQGARPFPFCWPLCEISRG
THNFSEELKIGEGGFGCVYRAVMRNTVYAVKRLKENADLEWTAVKQSFLT
EVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDRLHCQTQACP
PLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLTPKLGA
FGLARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTGRLAVDTDTF
SFGVVVLETLAGQRAVKTHGARTKYLKDLVEEEAEEAGVALRSTQSTLQA
GLAADAWAAPIAMQIYKKHLDPRPGPCPPELGLGLGQLACCCLHRRAKRR
PPMTQVYERLEKLQAVVAGVPGHWEAASCIPPSPQENSYVSSTGRAHSGA
APWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSC
PLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPP
QIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESD
EFQS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8914
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST tag for bacterial expression. This reference sequence differs to original at S196F and L532W.; ; Estimated MW of expressed protein : 104489 Daltons