cDNA Clone TIE2

• Plasmid: pGEX6P-1-TIE2 748-1101
• Parent Plasmid: pGEX6P1
• Expressed: GST-TIE2 748-1101
• Genbank: AAH35514.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCAATTGAAGAGAGCAAATGTGCAAAGGAGAATGGCCCAAGCCTT
CCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAACTCAGGGACTCTGG
CCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTACAATTTATCCAGTG
CTTGACTGGAATGACATCAAATTTCAAGATGTGATTGGGGAGGGCAATTT
TGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGGTTACGGATGGATG
CTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGATGATCACAGGGAC
TTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGACACCATCCAAACAT
CATCAATCTCTTAGGAGCATGTGAACATCGAGGCTACTTGTACCTGGCCA
TTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCTTCGCAAGAGCCGT
GTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATAGCACCGCGTCCAC
ACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGACGTGGCCCGGGGCA
TGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGATCTGGCTGCCAGA
AACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAGCAGATTTTGGATT
GTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAG
TGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGTGTACACAACCAAC
AGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGATTGTTAGCTTAGG
AGGCACACCCTACTGCGGGATGACTTGTGCTGAACTCTACGAGAAGCTGC
CCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTAT
GATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATGAGAGGCCATCATT
TGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAGGAGCGAAAGACCT
ACGTGAATACCACGCTTTATGAGAAGTTTACTTATGCAGGAATTGACTGT
TCTGCTGAAGAAGCGGCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQLKRANVQRRMAQAFQNVR
EEPAVQFNSGTLALNRKVKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVL
KARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLL
GACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSH
HLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQ
EVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPY
CGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQIL
VSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8894
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 67300 Daltons