cDNA Clone IRAK4

• Plasmid: pFBHTb IRAK4 D329A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-IRAK4 D329A
• Genbank: AAH13316.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAA
TGTTGGACTAATTAGGAAGCTGTCAGATTTTATTGATCCTCAAGAAGGAT
GGAAGAAGTTAGCTGTAGCTATTAAAAAACCATCTGGTGATGATAGATAC
AATCAGTTTCACATAAGGAGATTTGAAGCATTACTTCAAACTGGAAAAAG
TCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACAGTTG
GTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCAAGT
CTTTTGCTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAA
AGAAGCTATAACAGTTCAGCAAAAACAGATGCCTTTCTGTGACAAAGACA
GGACATTGATGACACCTGTGCAGAATCTTGAACAAAGCTATATGCCACCT
GACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTTAGTGATACACGTTT
TCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTTGATG
AACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGA
GTTGTATATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCT
TGCAGCAATGGTTGACATTACTACTGAAGAACTGAAACAGCAGTTTGATC
AAGAAATAAAAGTAATGGCAAAGTGTCAACATGAAAACTTAGTAGAACTA
CTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTAGTATATGTTTACAT
GCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACTCCAC
CACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGC
ATCAATTTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGC
AAATATCTTACTGGATGAAGCTTTTACTGCTAAAATATCTGGCTTTGGCC
TTGCACGGGCTTCTGAGAAGTTTGCCCAGACAGTCATGACTAGCAGAATT
GTGGGAACAACAGCTTATATGGCACCAGAAGCTTTGCGTGGAGAAATAAC
ACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATAATAA
CTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGAT
ATTAAAGAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGA
TAAAAAGATGAATGATGCTGATTCCACTTCAGTTGAAGCTATGTACTCTG
TTGCTAGTCAATGTCTGCATGAAAAGAAAAATAAGAGACCAGACATTAAG
AAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCTTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNKPITPSTYVRCLNVGLIRKL
SDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLF
DWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQ
KQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYE
LKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDIT
TEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLD
RLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEA
FTAKISAFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYS
FGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDAD
STSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8887
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression (kinase dead); ; Estimated MW of expressed protein : 54855 Daltons