cDNA Clone SAP97 (rat)

• Plasmid: pEBG-2T-SAP97 D397A
• Parent Plasmid: pEBG2T
• Expressed: GST-SAP97 D397A
• Genbank: U14950
• Tag: GST
• Species: Rat

Sequence of Insert

GCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGCGGTACCCC
GGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATC
GGTCGAAACTAAACCAAACCGAAGACAGACAGCTCAGGAGTTCCATAGAG
CGGGTTATTAGCATATTTCAGAGCAACCTCTTTCAGGCTTTAATAGACAT
TCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATC
ATTCAAAGCAGTGTGAACCGGTCCAACCCGGGAATCCTTGGGAGAGTGGC
AGCCTTTCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCCGGCGGCCTTAG
CCCTCCAGTAGAGAAATACCGGTATCAGGACGAAGAGGTGCTGCCTTCAG
AGCGGATTTCCCCGCAAGTCCCAAATGAGGTGCTGGGTCCGGAGCTGGTT
CACGTCTCGGAGAAGAGCCTGTCAGAGATTGAGAATGTCCACGGGTTTGT
CTCTCACTCTCATATCTCACCCATAAAGGCAAATCCTCCTCCTGTGCTGG
TCAACACAGACAGCTTGGAGACACCAACTTATGTTAATGGTACTGATGCA
GATTATGAATATGAGGAAATCACACTTGAAAGGGGAAATTCAGGCCTTGG
TTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAA
GTATTTTCATCACCAAAATTATCACGGGAGGAGCAGCTGCCCAGGATGGA
AGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGATGTTCG
TGATGTAACCCACAGCAAAGCAGTTGAAGCATTGAAAGAAGCTGGATCGA
TAGTGCGATTATATGTGAAAAGGCGGAAGCCAGCCTCAGAAAAAATCATG
GAAATAAAACTTATTAAAGGCCCTAAAGGTCTTGGGTTCAGCATTGCTGG
AGGTGTTGGAAACCAGCACATTCCTGGGGATAACAGCATCTATGTAACCA
AAATAATTGAAGGAGGTGCAGCACACAAGGACGGCAAACTTCAGATTGGA
GACAAGCTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAAGTTACTCATGA
AGAAGCAGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAG
TGGCAAAACCAACAAGTATGTATATAAATGATGGCTATGCACCACCCGAC
ATCACTAATTCTTCTTCTCAGTCTGTTGCCAACCATGTTAGCCCGTCTTC
ATACTTGGGCCAGACTCCAGCGTCACCAGCCAGATACTCACCCATTTCTA
AAGCAGTGCTTGGAGATGATGAGATCACTAGGGAACCTAGAAAAGTTGTT
CTTCATCGTGGCTCAACGGGACTTGGTTTCAACATTGTGGGAGGCGAAGA
TGGAGAAGGGATTTTTATCTCTTTCATCCTTGCTGGGGGACCTGCTGACC
TAAGCGGAGAGCTCAGAAAAGGAGATCGCATTATATCGGTAAACAGTGTT
GACCTCAGAGCTGCAAGTCATGAACAAGCAGCTGCCGCATTAAAAAACGC
AGGCCAAGCTGTCACTATTGTCGCACAGTATCGGCCTGAAGAGTACAGTC
GTTTTGAAGCTAAAATTCACGACTTGCGGGAGCAGATGATGAACAGCAGT
GTCAGCTCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAGCGGTCCCTCTA
TGTCAGAGCCCTTTTTGATTACGACAAGACTAAAGACAGTGGCCTTCCCA
GTCAAGGGTTGAACTTCAAATTCGGAGATATTCTCCATGTTATCAATGCT
TCTGATGACGAGTGGTGGCAGGCCAGACAGGTGACCCCAGATGGTGAGAG
TGACGAAGTTGGAGTAATTCCTAGTAAACGCAGAGTTGAGAAGAAAGAAC
GAGCCCGATTAAAAACAGTCAAATTCAATTCTAAAACAAGAGGAGATAAA
GGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTTCC
CTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGACC
AGCACGTAACTTCTAATGCCAGCGATAGCGAAAGTAGTTACCGTGGTCAA
GAAGAATATGTTTTATCGTATGAGCCGGTGAATCAGCAAGAAGTTAATTA
TACCCGACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATG
ACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACA
ACTAGACCAAAGCGTGACTATGAGGTGGATGGACGAGATTACCATTTTGT
GACTTCAAGAGAACAGATGGAGAAAGATATCCAGGAGCATAAATTCATTG
AAGCTGGCCAGTATAACAACCATCTATACGGAACGAGTGTCCAATCTGTG
CGAGCAGTAGCAGAGAAGGGCAAGCATTGTATCCTTGATGTGTCTGGAAA
TGCCATCAAGAGGTTGCAGATTGCACAGCTCTATCCAATATCTACTTTTA
TTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAACGCCTAACA
GACGAACAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGACTGGAGCAGGA
GTTCACTGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACA
TTTACAATCAAGTGAAGCAGATCATAGAAGAGCAGTCTGGTCCTTACATC
TGGGTCCCAGCAAAAGAAAAATTATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSGTIDAAASVMDICRIRPCGTPV
RKQDTQRALHLLEEYRSKLNQTEDRQLRSSIERVISIFQSNLFQALIDIQ
EFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSP
PVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVS
HSHISPIKANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGF
SIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRD
VTHSKAVEALKEAGSIVRLYVKRRKPASEKIMEIKLIKGPKGLGFSIAGG
VGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEE
AVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSSQSVANHVSPSSY
LGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDG
EGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAG
QAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSGSLRTSQKRSLYV
RALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESD
EVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGSFNDKRKKNLFSRKFPF
YKNKDQSEQETSDADQHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYT
RPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVT
SREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNA
IKRLQIAQLYPISTFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEF
TEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8885
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression. n.b. The current genbank reference differs greatly to the expressed protein.